Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,876,011	T→C	G113G (GGT→GGC)	nimT →	2‑nitroimidazole exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,011	0	T	C	100.0%	90.4 / NA	27	G113G (GGT→GGC)	nimT	2‑nitroimidazole exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

CCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTA  >  NC_000913/1875882‑1876135                                                                                                                                  |                                                                                                                             cccGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCggg                                                                                                                           >  1:265961/1‑133 (MQ=255)      tAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGa                                                                                                                          >  2:58769/1‑129 (MQ=255)      tAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGa                                                                                                                          <  1:58769/129‑1 (MQ=255)                ttttGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAAt                                                                                                            <  1:119964/133‑1 (MQ=255)                                     gTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGattaatt                                                                                       >  1:318085/1‑133 (MQ=255)                                     gTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGattaatt                                                                                       >  2:472867/1‑133 (MQ=255)                                      tttGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGattaatta                                                                                      >  1:492108/1‑133 (MQ=255)                                      tttGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGattaatta                                                                                      <  2:265961/133‑1 (MQ=255)                                              gTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGAt                                                                              <  2:293281/133‑1 (MQ=255)                                                         tCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTc                                                                   <  2:358015/133‑1 (MQ=255)                                                             tgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTc                                                               >  1:311312/1‑133 (MQ=255)                                                                       gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTa                                                     >  2:353380/1‑133 (MQ=255)                                                                       gCAATTCGCTCTCTCCCTTCGCCTGACTTATTCTTTGGCGGAACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTAACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTa                                                     <  2:143009/133‑1 (MQ=255)                                                                               ctctctCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCa                                             <  1:472867/133‑1 (MQ=255)                                                                                   ctcCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATAtt                                         >  2:92344/1‑133 (MQ=255)                                                                                                acttaTTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGGCGCCAGACTTACCGGCGCATATTCCCTGACAATggg                            >  2:14445/1‑133 (MQ=255)                                                                                                      tattTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGc                      >  1:80155/1‑133 (MQ=255)                                                                                                      tattTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGc                      >  1:259178/1‑133 (MQ=255)                                                                                                           ggCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCgg                 >  2:302669/1‑133 (MQ=255)                                                                                                           ggCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCgg                 >  2:448012/1‑133 (MQ=255)                                                                                                                  cAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCAc              <  1:368650/129‑1 (MQ=255)                                                                                                                  cAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCAc              >  2:368650/1‑129 (MQ=255)                                                                                                                  cAGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTggg          <  1:34812/133‑1 (MQ=255)                                                                                                                   aGCGGTCATTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGa         <  2:80155/133‑1 (MQ=255)                                                                                                                          aTTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGa         <  1:48380/126‑1 (MQ=255)                                                                                                                          aTTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGa         >  2:48380/1‑126 (MQ=255)                                                                                                                          aTTGGCGGCGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCGGCATCGCCACTGGAATCGGCTa  >  1:213296/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                             CCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTA  >  NC_000913/1875882‑1876135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑