Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,880,277	T→C	E161E (GAA→GAG)	leuE ←	leucine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,880,277	0	T	C	100.0%	89.0 / NA	28	E161E (GAA→GAG)	leuE	leucine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/10);  total (18/10)

CAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGC  >  NC_000913/1880166‑1880404                                                                                                                |                                                                                                                                cAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATa                                                                                                            <  1:455956/133‑1 (MQ=255)       aaTCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGTGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGTAGAAACTCTCCATCTCAAGCGTCGCGGCCAGAATAAAGAAt                                                                                                      >  2:146832/1‑133 (MQ=255)        aTCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATg                                                                                                     >  1:118928/1‑133 (MQ=255)        aTCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATg                                                                                                     <  2:23643/133‑1 (MQ=255)                  tGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGt                                                                                           >  2:70984/1‑133 (MQ=255)                      aaCTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTAtgt                                                                                       <  2:118928/133‑1 (MQ=255)                            aGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCa                                                                                 <  2:346189/133‑1 (MQ=255)                                aGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTaa                                                                             <  1:70984/133‑1 (MQ=255)                                                          cGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCTGTATGTGGGGCGTTAACATCGATGAACTGTACGCAAAACGac                                                   >  1:219624/1‑133 (MQ=255)                                                          cGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGac                                                   >  1:435963/1‑133 (MQ=255)                                                          cGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGac                                                   >  2:77731/1‑133 (MQ=255)                                                          cGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGac                                                   >  2:442484/1‑133 (MQ=255)                                                             gACAAAAGCACCAGATATTATCTGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGAcaca                                                <  1:146832/133‑1 (MQ=255)                                                               cAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATa                                              >  1:312358/1‑133 (MQ=255)                                                                                aTCAGGAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCg                             >  2:499969/1‑133 (MQ=255)                                                                                     gAAGCTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTa                        >  1:58023/1‑133 (MQ=255)                                                                                         cTCAAATAGCGGAAATTCACCAGCTCCAGCGTTGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCa                    <  2:176321/133‑1 (MQ=255)                                                                                         cTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCa                    >  1:19046/1‑133 (MQ=255)                                                                                         cTCAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCa                    <  2:435963/133‑1 (MQ=255)                                                                                           cAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAgg                  >  2:259559/1‑133 (MQ=255)                                                                                           cAAATAGCAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAgg                  <  2:121682/133‑1 (MQ=255)                                                                                                  cAGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCaaaa           >  1:295425/1‑133 (MQ=255)                                                                                                   aGAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAAt          >  2:79564/1‑133 (MQ=255)                                                                                                    gAAACTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCAGTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAAtt         >  1:103305/1‑133 (MQ=255)                                                                                                      aaCTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTaa       >  1:496899/1‑133 (MQ=255)                                                                                                       aCTCACCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAATTGGCTTTCGGATTAGTCAGGCGCAAAATTAAc      >  2:206377/1‑133 (MQ=255)                                                                                                        cTCACCAGCTCCAGCTTCCCCGACAGAATACAGAATGAAATTCCCTTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAAcg     <  1:149439/133‑1 (MQ=255)                                                                                                           aCCAGCTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAAcgcgc  >  1:96646/1‑133 (MQ=255)                                                                                                                |                                                                                                                                CAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGC  >  NC_000913/1880166‑1880404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑