Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,332	C→T	P163L (CCG→CTG)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,332	0	C	T	100.0%	62.2 / NA	19	P163L (CCG→CTG)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/12);  total (7/12)

TATTGGTATCACCCCTTTCCTGTCGCACATGGCAGAGCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCTGTGGCC  >  NC_000913/1886207‑1886439                                                                                                                              |                                                                                                            tATTGGTATCACCCCTTTCCTGTCGCACATGGCAGAGCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCt                                                                                                      <  2:467888/133‑1 (MQ=255)         tCACCCCTTTCCTGTCGCACATGGCAGAGCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGt                                                                                              >  1:118708/1‑133 (MQ=255)                          cacaTGGCAGAGCTGCAACACAGCGACGCCGATTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCAtcattcat                                                                             <  2:148085/133‑1 (MQ=255)                               ggCAGAGCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAAcc                                                                        >  2:93523/1‑133 (MQ=255)                                   gagCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGaa                                                                    <  1:93523/133‑1 (MQ=255)                                     gCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAaca                                                                  <  2:118708/133‑1 (MQ=255)                                               aGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAAt                                                        >  1:405064/1‑133 (MQ=255)                                                   aCGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAgc                                                    <  2:405064/133‑1 (MQ=255)                                                            ggCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGattatt                                           <  1:96943/133‑1 (MQ=255)                                                                 cTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCgg                                      <  1:481857/133‑1 (MQ=255)                                                                            cTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCt                           >  2:281266/1‑133 (MQ=255)                                                                              cgcgAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTgg                         >  1:440836/1‑133 (MQ=255)                                                                              cgcgAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATGAGCGCGAATATGGGGGGATATGGAACCTgg                         >  1:135817/1‑133 (MQ=255)                                                                                  aaaTCCAGAATGTTGCGCATTTCGCGATCAGCCAGTCCAGCGTCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGcaca                     <  2:160799/133‑1 (MQ=255)                                                                                  aaaTCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGcaca                     <  2:298327/133‑1 (MQ=255)                                                                                   aaTCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGcacac                    <  1:281266/133‑1 (MQ=255)                                                                                   aaTCCAGAAAGTTGCGCATTTAGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGcacac                    <  1:453711/133‑1 (MQ=255)                                                                                                 cgcATTTCGTGATGAGCTAGTCCAGCATCTGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCtgt      >  1:16332/1‑133 (MQ=255)                                                                                                     tttCGTGACGAGCTAATCCAGCATCTGCAGACTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCTGTGGcc  <  1:228566/133‑1 (MQ=255)                                                                                                                              |                                                                                                            TATTGGTATCACCCCTTTCCTGTCGCACATGGCAGAGCTGCAACACAGCGACGTCGACTGGCAGCTACATTACTGCTCGCGAAATCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCTGTGGCC  >  NC_000913/1886207‑1886439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑