Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,894,728 A→G F2L (TTT→CTT)  yoaH ← UFP0181 family protein YoaH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,894,7280AG100.0% 103.8 / NA 30F2L (TTT→CTT) yoaHUFP0181 family protein YoaH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

GGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCT  >  NC_000913/1894603‑1894841
                                                                                                                             |                                                                                                                 
ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGtt                                                                                                            >  2:22253/1‑133 (MQ=255)
ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGtt                                                                                                            >  2:442430/1‑133 (MQ=255)
     gCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTc                                                                                                       <  1:266755/133‑1 (MQ=255)
     gCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTc                                                                                                       <  1:442430/133‑1 (MQ=255)
     gCAGCTCTACTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTc                                                                                                       <  1:69637/133‑1 (MQ=255)
                 ccaccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAAttgt                                                                                           >  2:487533/1‑133 (MQ=255)
                     caGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGgc                                                                                       >  2:278500/1‑133 (MQ=255)
                      aGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGgcg                                                                                      <  1:323999/133‑1 (MQ=255)
                       gcgcGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGgcgc                                                                                     <  1:487533/133‑1 (MQ=255)
                        cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTACCTCCTGCTAAATTATTGGCGCt                                                                                    >  1:223110/1‑133 (MQ=255)
                        cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCt                                                                                    >  1:58877/1‑133 (MQ=255)
                            aTTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTTatt                                                                                >  1:427559/1‑133 (MQ=255)
                                             cATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGc                                                               <  1:338996/133‑1 (MQ=255)
                                               tCCCTTGTGCAATCAGTTCCTGGATACGCCCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTCACCCTCCACGCATTGTTAACTCCTGCCAAATTGTTGGCGCTAATTATTTCACGCTACCCGGccc                                                             <  2:322107/133‑3 (MQ=255)
                                                  cTTGTGCCATTAGTTCCTGGATCCTCTCTACAGCTTTTTGCCGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATa                                                          <  2:177713/133‑1 (MQ=255)
                                                        ccATCAGTTCTTGGATCCGCTCGATAGCTTTTCGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                    <  2:144078/133‑1 (MQ=255)
                                                        ccATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                    <  2:58877/133‑1 (MQ=255)
                                                        ccATCAGTCCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                    <  1:410625/133‑1 (MQ=255)
                                                          aTCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTAGCCGGCACATCGCCAGTag                                                  >  1:139861/1‑133 (MQ=255)
                                                             aGTTCCTGGATCCGCTCTACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGt                                               >  1:272431/1‑133 (MQ=255)
                                                                  cTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTTATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGa                                          <  2:427559/133‑1 (MQ=255)
                                                                           cTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAg                                 >  1:7375/1‑133 (MQ=255)
                                                                                    tttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTAtct                        <  1:22253/133‑1 (MQ=255)
                                                                                    tttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTAtct                        <  2:272431/133‑1 (MQ=255)
                                                                                        tgctgcTGTTCATGGGTGAGTGAAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCg                    <  2:347696/133‑1 (MQ=255)
                                                                                             ctgTTCATGGGTGAGCGAAGGTTAACCTGCCAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCtgtg               <  1:234342/133‑1 (MQ=255)
                                                                                                          agtgaAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGATGACTTTACCTCCCGCTATGATTACTTTActct  >  2:168317/1‑133 (MQ=255)
                                                                                                          agtgaAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct  >  2:126316/1‑133 (MQ=255)
                                                                                                          agtgaAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct  >  2:122043/1‑133 (MQ=255)
                                                                                                          agtgaAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct  >  1:64838/1‑133 (MQ=255)
                                                                                                          agtgaAGGTAAACCTGCAAGCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct  >  2:64960/1‑133 (MQ=255)
                                                                                                                             |                                                                                                                 
GGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCT  >  NC_000913/1894603‑1894841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑