Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,391	T→G	T399P (ACC→CCC)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,391	0	T	G	100.0%	62.9 / NA	20	T399P (ACC→CCC)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/5);  total (15/5)

CTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCG  >  NC_000913/1900262‑1900519                                                                                                                                  |                                                                                                                                 cTTTTACCAGGCAGCCGTCACCATCAGTAATGTTTTCCGGCATTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCAc                                                                                                                               <  2:221835/133‑1 (MQ=255)           ccagccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAg                                                                                                                     >  2:401723/1‑133 (MQ=255)                         cAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCacg                                                                                                       >  1:261823/1‑133 (MQ=255)                                 aTTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACgcagca                                                                                               <  2:257412/133‑1 (MQ=255)                                      cGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGTGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGccc                                                                                          >  2:56938/1‑133 (MQ=255)                                      cGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGccc                                                                                          >  2:163734/1‑133 (MQ=255)                                        gCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCaa                                                                                        <  2:261823/133‑1 (MQ=255)                                                         gggAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGa                                                                       >  2:275197/1‑133 (MQ=255)                                                              ttCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGtt                                                                  >  2:92983/1‑133 (MQ=255)                                                                   ccGCAATGGCTTCCATCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCgg                                                             <  1:163734/133‑1 (MQ=255)                                                                                   cacaTCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGga                                             <  1:56938/133‑1 (MQ=255)                                                                                           gcggcgTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGa                                     >  1:341861/1‑133 (MQ=255)                                                                                               cgTGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCa                                 >  1:464872/1‑133 (MQ=255)                                                                                                 tGACCAGACCTTGTACCACACCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAAt                               >  2:96212/1‑133 (MQ=255)                                                                                                                  acacCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGc              >  1:126755/1‑133 (MQ=255)                                                                                                                   cacCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGGCGCCGAAGCAAACGTCGCCACTTCAACGcc             >  1:183544/1‑133 (MQ=255)                                                                                                                      cAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATGGCCGGAGAGGCCTATGGAATCGCCGACACAATAACGCCctc          >  2:223267/1‑133 (MQ=255)                                                                                                                      cAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCctc          >  1:454488/1‑133 (MQ=255)                                                                                                                        aaCTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCCAAGCAATCGCCTCCCCATCAAAGCCCTCtt        >  1:204625/1‑133 (MQ=255)                                                                                                                          cTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTcc      >  2:431699/1‑133 (MQ=255)                                                                                                                              ttGGGCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAgcg  >  2:449365/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                 CTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCG  >  NC_000913/1900262‑1900519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑