Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,902,757	G→A	G237D (GGC→GAC)	manX →	mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,902,757	0	G	A	100.0%	91.6 / NA	28	G237D (GGC→GAC)	manX	mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

TTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAC  >  NC_000913/1902628‑1902884                                                                                                                                  |                                                                                                                                ttAGTGATGAGGTGGATGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGa                                                                                                                              <  2:217553/133‑1 (MQ=255) ttAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGa                                                                                                                              >  1:327125/1‑133 (MQ=255)   aGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAGAATGATTCACGTGTACTACAACCCGGAATAGGCTGATGAAc                                                                                                                            >  2:130347/1‑133 (MQ=255)   aGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAAc                                                                                                                            >  2:110739/1‑133 (MQ=255)   aGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAAAAACCCGTAATATGCTGACGAAc                                                                                                                            >  1:226441/1‑133 (MQ=255)     tgatgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAGTGATTCGCTTCTACAAGTACCCGAATTATGCTGACGAAcac                                                                                                                          >  2:242927/1‑131 (MQ=255)      gatgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAAcgcg                                                                                                                         <  2:397855/133‑1 (MQ=255)      gatgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAAcgcg                                                                                                                         <  2:298524/133‑1 (MQ=255)        tgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTa                                                                                                                       >  2:310657/1‑133 (MQ=255)        tgaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTa                                                                                                                       <  2:1355/133‑1 (MQ=255)         gaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTaa                                                                                                                      >  2:354123/1‑133 (MQ=255)         gaAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTaa                                                                                                                      <  2:14448/133‑1 (MQ=255)             tGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCt                                                                                                                  <  2:280108/133‑1 (MQ=255)                   cGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAACAAGCTGACGAACCCGTAATGCTGTTAtt                                                                                                            >  2:225254/1‑133 (MQ=255)                          cGTTCGTAAGACACTCCTCACCCAGGTTCCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTAccaac                                                                                                     <  2:147550/133‑1 (MQ=255)                              cGTAAGACCCTGCTCCCCAAGGTTGCGCCTTCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGATGAACGCGTAATGCTGTTATTTAccaacccaa                                                                                                 <  1:130347/133‑1 (MQ=255)                               gTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGAGGAATGCGTAATGCTGTCATTTGccaacccaac                                                                                                >  2:158913/1‑133 (MQ=255)                                              cccAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTAATTACCAACCCAATAGATTTAGAGCGtct                                                                                 >  2:205881/1‑133 (MQ=255)                                                      gCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAg                                                                         <  2:366787/133‑1 (MQ=255)                                                                              gTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTa                                                 >  2:306205/1‑133 (MQ=255)                                                                                  ttGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGt                                             <  1:354123/133‑1 (MQ=255)                                                                                          gCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTa                                     >  2:377970/1‑133 (MQ=255)                                                                                           ccAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTAt                                    <  2:327125/133‑1 (MQ=255)                                                                                                     tCGCGTCTACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGt                          <  2:402984/133‑1 (MQ=255)                                                                                                      cgcgTCTACAACAACCCGGAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTc                         <  1:225254/133‑1 (MQ=255)                                                                                                       gcgTCTACGACAACCTGACATACGCTGACGCACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCa                        <  1:242927/133‑1 (MQ=255)                                                                                                       gcgTCTACAACAACCCGAAATATGCTTACGAAAGCGTAATGGTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCCCCTCTGTTAACGTCGGTGGTATGGCATTCCGTCa                        <  2:226441/133‑1 (MQ=255)                                                                                                            tACAACAACCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTa                   >  2:289712/1‑133 (MQ=255)                                                                                                                   acCCGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCa            <  1:110739/133‑1 (MQ=255)                                                                                                                     ccGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAgg          >  1:460456/1‑133 (MQ=255)                                                                                                                     ccGAAATATGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTAGTATGGCATTCAGTTAGGGTACAACCCAgg          >  1:191148/1‑133 (MQ=255)                                                                                                                             tGCTGACGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAc  >  2:267437/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                TTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTCCGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTAATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGGTATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAAC  >  NC_000913/1902628‑1902884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑