Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,205	T→C	V173A (GTG→GCG)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,205	0	T	C	100.0%	45.1 / NA	14	V173A (GTG→GCG)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/11);  total (3/11)

GCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAA  >  NC_000913/1906096‑1906282                                                                                                              |                                                                              gCATTGGCTATTGGTTGTGCAATCTTGATTCTGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGa                                                        <  1:223252/133‑1 (MQ=255)            tGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACg                                             <  2:440004/133‑1 (MQ=255)                 gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTCGCGCTGATGGGCGTCGTCGGCCAGAACCTCTGGACGCACtt                                        >  2:201558/1‑133 (MQ=255)                    cAACCTTGATTATGTTAACATTAGGGATTATGGTTGGTCGCCTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCa                                     <  2:175736/133‑1 (MQ=255)                    cAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCa                                     <  2:426093/133‑1 (MQ=255)                     aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCAc                                    >  2:466167/1‑133 (MQ=255)                                   cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACg                      <  1:466167/133‑1 (MQ=255)                                      cATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTg                   >  1:73037/1‑133 (MQ=255)                                            ggATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCagag             <  2:275650/133‑1 (MQ=255)                                            ggATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCagag             <  2:35665/133‑1 (MQ=255)                                              atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt           <  2:29900/133‑1 (MQ=255)                                              atgatgGTGGGTCGCTTTCTCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt           <  1:227105/133‑1 (MQ=255)                                              atgatgGTAGGTCGCATTTTCGCCTCAATTCTTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt           <  1:201558/133‑1 (MQ=255)                                                gatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGa         <  1:14905/133‑1 (MQ=255)                                                       ggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGCGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaa  >  1:324858/1‑133 (MQ=255)                                                                                                              |                                                                              GCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAA  >  NC_000913/1906096‑1906282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑