| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 135,923 | C→T | C181Y (TGC→TAC) | speE ← | spermidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 135,923 | 0 | C | T | 100.0% | 43.0 / NA | 15 | C181Y (TGC→TAC) | speE | spermidine synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/6); total (9/6) | |||||||||||
GGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCAT > NC_000913/135795‑136046 | ggATCGCCGCCTGATAGAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGGTACTGCCAGGATTCAGGTAACg > 1:194131/1‑133 (MQ=255) gccgccTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGc < 2:62375/133‑1 (MQ=255) aCGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACGGCCAGGATTCAGCTAACGTTTGCAGCCTTCGTAAAATGCCg > 2:173229/1‑133 (MQ=255) cGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGa > 2:287367/1‑133 (MQ=255) cGTCGCTGAAGTAATGGCCGAGTTTGCGATAGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGa < 1:173229/133‑1 (MQ=255) gCGATGGCTGTCGATGGCTTTTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCg > 2:75313/1‑133 (MQ=255) cTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCggg < 1:396466/122‑1 (MQ=255) cTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCggg > 2:396466/1‑122 (MQ=255) tGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCa < 1:287367/133‑1 (MQ=255) aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGTAGTCGGAGATAATGACATCaaa < 2:434425/133‑1 (MQ=255) acgaAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTaa > 1:460348/1‑133 (MQ=255) acgaAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTaa < 2:460348/133‑1 (MQ=255) cGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGAc > 2:270803/1‑133 (MQ=255) cAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTAATAAACGAAATTGACGcc > 1:194386/1‑133 (MQ=255) ggATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCAt > 2:49837/1‑133 (MQ=255) | GGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCAT > NC_000913/135795‑136046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |