Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,360	T→C	G91G (GGT→GGC)	fliN →	flagellar motor switch protein FliN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,360	0	T	C	100.0%	78.3 / NA	25	G91G (GGT→GGC)	fliN	flagellar motor switch protein FliN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

CGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCG  >  NC_000913/2021231‑2021479                                                                                                                                  |                                                                                                                        cGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTg                                                                                                                      >  2:91751/1‑133 (MQ=255)    aaCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTggcg                                                                                                                   >  1:125792/1‑133 (MQ=255)      cGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTggcggg                                                                                                                 >  2:265753/1‑133 (MQ=255)        ttGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTggcgggcg                                                                                                               <  1:91751/133‑1 (MQ=255)         tGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGa                                                                                                              >  1:404854/1‑133 (MQ=255)            aGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAAcc                                                                                                           <  2:404854/133‑1 (MQ=255)                 atCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTgg                                                                                                      <  1:76038/133‑1 (MQ=255)                 atCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTgg                                                                                                      <  1:9234/133‑1 (MQ=255)                   cGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACGACTGGat                                                                                                    >  2:427204/1‑133 (MQ=255)                     aCCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGatat                                                                                                  >  1:436400/1‑133 (MQ=255)                      ccTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATAtt                                                                                                 >  1:77383/1‑133 (MQ=255)                            tATGGATATTCCGGTCAGGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATc                                                                                           <  2:71263/133‑1 (MQ=255)                              tGGATATTCCCGTCACGCTGACCGCCGATCTGTGCCGTACGAGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCaa                                                                                         <  2:193966/133‑1 (MQ=255)                                    ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGGACCACTGGATATTCTGATCAACGGTTa                                                                                   >  1:110514/1‑133 (MQ=255)                                    ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTa                                                                                   >  1:421373/1‑133 (MQ=255)                                          tCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAAt                                                                             >  1:449713/1‑133 (MQ=255)                                              gCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGcc                                                                         <  1:265753/133‑1 (MQ=255)                                                   ccGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAggg                                                                    <  2:125792/133‑1 (MQ=255)                                                     gTCGAGCTGGGCCGTACGCGGATGACAATCAAGGAGCTGTGGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCg                                                                  <  2:436400/133‑1 (MQ=255)                                                                        ggATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGa                                               <  2:449713/133‑1 (MQ=255)                                                                                  cAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACGACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGc                                     <  1:427204/133‑1 (MQ=255)                                                                                         cTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGAGGGGCGTTGCCCATAAATATGGCGTggggg                              >  1:175782/1‑132 (MQ=255)                                                                                          tGTTGCGTCTTACGCCAGGGTCCGTCGTGGCGTTGGACGGCCTGGCGGGGGAACCCCTGGATATTCTGATCAACGGTTATTTAACCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGAt                             <  2:175782/133‑1 (MQ=255)                                                                                           gTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATc                            >  2:301108/1‑133 (MQ=255)                                                                                            ttGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCa                           >  2:337105/1‑133 (MQ=255)                                                                                                gTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa                       <  1:370512/133‑1 (MQ=255)                                                                                                gTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa                       <  2:421373/133‑1 (MQ=255)                                                                                                gTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa                       <  1:313515/133‑1 (MQ=255)                                                                                                  cTGACGCAAGGGTCCGTCGTGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGata                     <  1:337105/133‑1 (MQ=255)                                                                                                                     tGGCGCTGGACGGCCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAACTATGGCGTAGGGATCAACCATATTAATACTCCGTCTGAGCg  >  2:192481/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                        CGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCG  >  NC_000913/2021231‑2021479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑