Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,373	T→C	L136L (TTA→CTA)	rcsA →	DNA‑binding transcriptional activator RcsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,373	0	T	C	100.0%	113.0 / NA	34	L136L (TTA→CTA)	rcsA	DNA‑binding transcriptional activator RcsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

CTATTGGTCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGT  >  NC_000913/2024250‑2024501                                                                                                                            |                                                                                                                                 cTATTGGTCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACC‑CGTTTTTAAATATGCCGACGCTATCATTGAg                                                                                                                        <  1:501002/133‑1 (MQ=255)        tCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTACAAATGCCGACGCAATCAAGGAGccgagc                                                                                                                  >  2:250777/1‑131 (MQ=255)        tCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGccgaac                                                                                                                  >  2:96062/1‑133 (MQ=255)        tCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGccgaac                                                                                                                  >  2:282683/1‑133 (MQ=255)          aGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGccgaaccg                                                                                                                >  2:480977/1‑133 (MQ=255)                                       gATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCa                                                                                   <  1:417357/133‑1 (MQ=255)                                        aTTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAg                                                                                  >  2:52746/1‑133 (MQ=255)                                        aTTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAg                                                                                  >  1:104775/1‑133 (MQ=255)                                        aTTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATGGAGCCGAAACGAATCGAATATGTTGCGAATGTGGATGGCAg                                                                                  >  2:251404/1‑133 (MQ=255)                                                 gAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGaa                                                                         <  1:454443/133‑1 (MQ=255)                                                 gAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGaa                                                                         <  2:104775/133‑1 (MQ=255)                                                             gattaccTTGGCGAAATTCTTAAAAAAGAGACAACGATAACCTCGTTTTTAAAAATGCCGACGCTAACATTGAGCCGAACCGAATCGAGTATGGTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAAtct                                                             <  2:263495/128‑1 (MQ=255)                                                                  ccTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGAc                                                        <  2:415599/133‑1 (MQ=255)                                                                      ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGATCaaa                                                    >  1:437417/1‑133 (MQ=255)                                                                      ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCaaa                                                    >  2:385115/1‑133 (MQ=255)                                                                             ttCTGAAAAAAGAGACAACGTTAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACTGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAatat                                             >  2:116488/1‑133 (MQ=255)                                                                              tCTGAAAAAAGAGACCACTATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATc                                            <  1:251404/133‑1 (MQ=255)                                                                                     aaaGAGACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCa                                     >  2:496679/1‑133 (MQ=255)                                                                                       agagACAACGATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGTGAACCATTCAAATCACTAACCAAATAAATATCAAAGCCAAg                                   >  2:238986/1‑133 (MQ=255)                                                                                                gATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCa                          <  1:385115/133‑1 (MQ=255)                                                                                                gATAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCa                          <  1:282683/133‑1 (MQ=255)                                                                                                gATAACCTAGTTTTTCAATATGCCGACGCTTTCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCa                          <  1:238986/133‑1 (MQ=255)                                                                                                 aTAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCAt                         <  1:509459/133‑1 (MQ=255)                                                                                                 aTAACCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCAt                         <  2:84913/133‑1 (MQ=255)                                                                                                   aaCCTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCg                       >  1:504131/1‑133 (MQ=255)                                                                                                      cTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGATCAAATGAATATCAAAGCCAAGACCGTTTCATCGCAt                    <  2:437417/133‑1 (MQ=255)                                                                                                      cTCGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCAt                    <  1:13050/133‑1 (MQ=255)                                                                                                       tCGTTTTTGAATATGTCGCCGCCATCATTGTGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATa                   <  1:169508/133‑1 (MQ=255)                                                                                                        cGTTTTTAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATaa                  <  2:489258/133‑1 (MQ=255)                                                                                                          tttttAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAg                >  2:101523/1‑133 (MQ=255)                                                                                                           ttttAAATTTGCCGATGCTATCTTTGAGCCAAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAgg               <  1:187483/133‑1 (MQ=255)                                                                                                            tttAAATATGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGt              <  2:461214/133‑1 (MQ=255)                                                                                                                  tatGCCGAGGCTATCATTGAGCTGAACCGACTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATAtt        <  1:212342/133‑1 (MQ=255)                                                                                                                    tGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGATCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTaa      >  1:482111/1‑133 (MQ=255)                                                                                                                    tGCCGACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTaa      >  2:497694/1‑133 (MQ=255)                                                                                                                        gACGCTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGt  <  2:294045/133‑1 (MQ=255)                                                                                                                            |                                                                                                                                 CTATTGGTCAGAAAAAATTTATTGATCAGTTCTAAATCGATTAAACCGGAATCTCTCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGT  >  NC_000913/2024250‑2024501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑