Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,062,074	G→A	intergenic (‑141/‑186)	nac ← / → asnV	DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,062,074	0	G	A	100.0%	78.9 / NA	24	intergenic (‑141/‑186)	nac/asnV	DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/14);  total (10/14)

GCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATG  >  NC_000913/2061958‑2062201                                                                                                                     |                                                                                                                                gCCCAAGTGCCGCCATTACTTACACCCACATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTg                                                                                                                 <  2:146028/133‑1 (MQ=255)      aGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTcgcg                                                                                                            <  1:427970/133‑1 (MQ=255)           cgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTccc                                                                                                       <  2:107513/133‑1 (MQ=255)                 tacttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGccc                                                                                                 <  1:392702/133‑1 (MQ=255)                   cttacAACCAGATTGCAAGATGCTGGCCAGTTGTATTTTGGGGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCta                                                                                               <  2:226242/133‑1 (MQ=255)                             gATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAaggagg                                                                                     >  2:505749/1‑133 (MQ=255)                                tGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTa                                                                                  <  1:72050/133‑1 (MQ=255)                                  cAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATg                                                                                <  1:275802/133‑1 (MQ=255)                                          ttGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGa                                                                        >  2:69783/1‑133 (MQ=255)                                                  tttATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCAt                                                                <  1:505749/133‑1 (MQ=255)                                                   ttATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCAtt                                                               >  1:434133/1‑133 (MQ=255)                                                    tATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTa                                                              <  2:434133/133‑1 (MQ=255)                                                                     aCAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAg                                             <  2:423237/133‑1 (MQ=255)                                                                        aGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTc                                          >  2:312170/1‑133 (MQ=255)                                                                          cTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTcac                                        <  1:69783/133‑1 (MQ=255)                                                                           tAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTGCATTACCCAATAAGATTGAAAGCTcacc                                       >  2:216680/1‑133 (MQ=255)                                                                            aaCCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTcacca                                      <  2:497134/133‑1 (MQ=255)                                                                                 aCTGTCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTttg                                 >  1:76268/1‑133 (MQ=255)                                                                                     tCAAATAAGAGATTATGATAGATTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGa                             >  2:433014/1‑133 (MQ=255)                                                                                                          aTTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGcaaacaaacaaa        >  2:125047/1‑133 (MQ=255)                                                                                                          aTTCGTCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCAACACGTTGCTGAAATTGTCAGcaaacaaacaaa        >  2:162878/1‑133 (MQ=255)                                                                                                               tCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAAt   >  2:497804/1‑133 (MQ=255)                                                                                                               tCATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAAt   <  2:76268/133‑1 (MQ=255)                                                                                                                cATTTACTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATg  <  1:433014/133‑1 (MQ=255)                                                                                                                     |                                                                                                                                GCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATG  >  NC_000913/2061958‑2062201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑