Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,678	T→C	V308A (GTG→GCG)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,678	0	T	C	100.0%	78.9 / NA	24	V308A (GTG→GCG)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/9);  total (15/9)

GAGACATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATT  >  NC_000913/2083552‑2083807                                                                                                                               |                                                                                                                                  gagaCATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGc                                                                                                                             >  1:480157/1‑133 (MQ=255)      aTTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAgg                                                                                                                        <  1:7453/133‑1 (MQ=255)                                cGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGcc                                                                                              >  2:29446/1‑133 (MQ=255)                                cGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCACAGGCGAGTACTCGGCGCCCGCAAGATGcc                                                                                              >  1:227098/1‑133 (MQ=255)                                     caTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGccgaccg                                                                                         >  1:474837/1‑133 (MQ=255)                                           gcgAGCGCTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTggg                                                                                   <  1:433361/133‑1 (MQ=255)                                                     atataCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGt                                                                         <  1:104759/133‑1 (MQ=255)                                                     atataCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGt                                                                         <  1:29446/133‑1 (MQ=255)                                                          ccGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCa                                                                    >  2:686/1‑133 (MQ=255)                                                           cGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTc                                                                        <  1:371072/128‑1 (MQ=255)                                                           cGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTc                                                                        >  2:371072/1‑128 (MQ=255)                                                             cAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGGCGACCGACTGGGAAATAATCGACAGCATTg                                                                 >  1:172391/1‑133 (MQ=255)                                                             cAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTg                                                                 >  1:56090/1‑133 (MQ=255)                                                             cAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTg                                                                 >  1:33677/1‑133 (MQ=255)                                                                aaaCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCt                                                              >  1:274788/1‑133 (MQ=255)                                                                     gATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATa                                                         <  2:480157/133‑1 (MQ=255)                                                                      aTCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATaa                                                        <  2:474837/133‑1 (MQ=255)                                                                                     ccgccgTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCg                                         >  1:67368/1‑133 (MQ=255)                                                                                                       tGGTGCATATCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTgcg                       <  1:439787/133‑1 (MQ=255)                                                                                                               atCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAg               >  1:473839/1‑133 (MQ=255)                                                                                                               atCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAg               >  1:423175/1‑133 (MQ=255)                                                                                                                tCAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAgt              >  2:112081/1‑133 (MQ=255)                                                                                                                 cAATAAATGTCCGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAgtg             <  2:473839/133‑1 (MQ=255)                                                                                                                            cGGCGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATAtt  >  2:474090/1‑133 (MQ=255)                                                                                                                               |                                                                                                                                  GAGACATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATT  >  NC_000913/2083552‑2083807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑