| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 140,467 | C→T | E253E (GAG→GAA) | gcd ← | quinoprotein glucose dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 140,467 | 0 | C | T | 95.7% | 71.2 / NA | 23 | E253E (GAG→GAA) | gcd | quinoprotein glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/11); minor base A (0/1); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCAC > NC_000913/140339‑140591 | tcatTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGGGCTGGAAAGACACGATGGTTttcagttcag > 1:169990/1‑133 (MQ=255) cGGAAGAATGATACGACGCGGGCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTaa < 1:278573/133‑1 (MQ=255) cGGAAGAAAGATACGCCGAGGGCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTaa < 1:156731/133‑1 (MQ=255) ggCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCt > 1:433476/1‑133 (MQ=255) gCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGCTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTg > 1:511101/1‑133 (MQ=255) aTCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTggcg < 2:433476/133‑1 (MQ=255) aCTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCAt > 1:110471/1‑127 (MQ=255) aCTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCAt < 2:110471/127‑1 (MQ=255) aaGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGACATTTCTCGTTGGCGCGGGCGGCATGAAGCGCAAACAGgcg > 2:143823/1‑133 (MQ=255) tCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAg < 2:110293/133‑1 (MQ=255) tCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAg < 2:339400/133‑1 (MQ=255) tttGGCTTCATGATACGAGACACCTCGGCAGGTTACGTGCTCGAAAGACTCGTTGGTTTTCAGTTCAGGCTCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTa < 2:169990/133‑1 (MQ=255) tttGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTa < 2:511101/133‑1 (MQ=255) tGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTaca < 2:338703/133‑1 (MQ=255) acacCACGTCAGGTTCCGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGc < 1:252766/133‑1 (MQ=255) acacCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGc > 1:53545/1‑133 (MQ=255) cGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACtt > 2:424063/1‑133 (MQ=255) gCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGGGCTGGTGAACGGGACAAAGGTAAAGAGTGTCGCCCACTTTa > 2:144522/1‑133 (MQ=255) gCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGGGTCGCACACTTTa > 2:98485/1‑133 (MQ=255) cAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTaa < 1:412617/133‑1 (MQ=255) tACGTGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGc > 2:379337/1‑133 (MQ=255) tGCTGGAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCa < 2:53545/133‑1 (MQ=255) gAAAGACTCGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCa > 2:375429/1‑133 (MQ=255) cGTTGGTTTTCAGTTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCGCACTTTAATCGGCGACACTTCGTTGGTGAt > 1:134342/1‑133 (MQ=255) ttGGTTTTTAGTTCACGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGAttt < 1:143823/133‑1 (MQ=255) gTTTTCAGATCAGGAGCGTAATCCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCAc < 1:169592/133‑1 (MQ=255) | TCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACTTCCGGCGAAGCGGTTTCTGCTTTGGCTTCATGATAAGAGACACCACGGCAGGTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCAC > NC_000913/140339‑140591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |