Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,084,598	T→C	M310V (ATG→GTG)	yeeE ←	inner membrane protein YeeE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,084,598	0	T	C	100.0%	87.1 / NA	27	M310V (ATG→GTG)	yeeE	inner membrane protein YeeE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

AATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCATAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACT  >  NC_000913/2084469‑2084714                                                                                                                                  |                                                                                                                     aaTTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACGAGGCCAATGCAGGCCTGCCAGGTCATCATTACAGTCACAAc                                                                                                                   >  2:140734/1‑133 (MQ=255)   tttGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCa                                                                                                                 <  2:78143/133‑1 (MQ=255)     tGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGt                                                                                                               <  1:120348/133‑1 (MQ=255)     tGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGt                                                                                                               <  1:415509/133‑1 (MQ=255)     tGCCGCAGCAGTTGCCAGCCGCACCTTACGTTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGt                                                                                                               <  1:140734/133‑1 (MQ=255)      gCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATACATACAAGGCAAATCCAGCTCTGTCAGGCCATCATTACAGTCACAACCAGTc                                                                                                              >  2:191730/1‑133 (MQ=255)       ccGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGTCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCTATCCAGCCATGCGAGGGCATCATTGGAGTCTCAACCAGTcc                                                                                                             >  2:139677/1‑133 (MQ=255)               gTTGCCAGTCGCGCCGTAAGCTCCGGTCGAGCATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGa                                                                                                     <  1:248725/133‑1 (MQ=255)                     aGTCGCGCTTTACGCTGCTGTCCAACATACTCAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGc                                                                                               <  1:252078/133‑1 (MQ=255)                         gcgcCTTACGCTGCGGTCAAACGTACACAATCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAacc                                                                                           <  1:151636/133‑1 (MQ=255)                          cgcCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAATCCATTACCGATAGAGCAacca                                                                                          >  1:179100/1‑133 (MQ=255)                               tccGCTGCGGTCGAACATACCCAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGc                                                                                     <  2:57792/131‑1 (MQ=255)                                          cGAACATACAGAAGCCAGGACGCAGTCCCGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCAc                                                                          <  2:182022/133‑1 (MQ=255)                                                 acacAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGcc                                                                   <  2:122009/133‑1 (MQ=255)                                                     aaGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATc                                                               <  1:71447/133‑1 (MQ=255)                                                      aGCCAGGACGCAGTCTAGACTCCGTGAATCATGAATACAAGTCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCa                                                              <  2:179100/133‑1 (MQ=255)                                                       gCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAGCCAGCTGGAATACTGGGACCGCAGCCCATCAg                                                             >  2:150146/1‑133 (MQ=255)                                                       gCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAg                                                             >  1:315160/1‑133 (MQ=255)                                                          aGGACGCATTCCAGATTCCGAGCATCATAACTACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTac                                                          <  1:139677/133‑1 (MQ=255)                                                                       gACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTATCCCg                                             <  2:380940/133‑1 (MQ=255)                                                                             gagaATCACAAATACAAGACCATTCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGt                                       <  1:249166/133‑1 (MQ=255)                                                                                        aTACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGttgtttg                            >  1:445369/1‑133 (MQ=255)                                                                                             aGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCAt                       >  2:89448/1‑133 (MQ=255)                                                                                                   aTCCAGCCCTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGACACCGAAGCCCATCAGTACACCACCTAACGCGCTACGAAATGTTTTTTGTGCATCAGCTg                 >  1:228169/1‑133 (MQ=255)                                                                                                       aGCCCTGCCAGGTCAACACTGCCGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGcgcg             <  1:150146/133‑1 (MQ=255)                                                                                                         cccTGCCAGGTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGaa           >  1:424911/1‑133 (MQ=255)                                                                                                                  gTCATCATTGCAGTCACAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACt  >  2:452195/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                     AATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGCCAATCCAGCCCTGCCAGGTCATCATTGCAGTCATAACCAGTCCATTACCGATAGAGCAACCACCTGCAATACTGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACT  >  NC_000913/2084469‑2084714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑