Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,112,050 T→C T338A (ACA→GCA)  rfbB ← dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,112,0500TC97.7% 145.6 / ‑3.3 43coding (1012/1086 nt)rfbBdTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (24/18);  minor base . (0/1);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAG  >  NC_000913/2111925‑2112178
                                                                                                                             |                                                                                                                                
gCTGTAGTTCACAACCTACCTGCCATGTTTTGTCAAAAAGGGGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGAc                                                                                                                           <  2:158207/133‑1 (MQ=255)
     aGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTa                                                                                                                      <  1:36429/133‑1 (MQ=255)
            aaCCTACCTGCCCTTTTTTGCCAAAAAGGAGGATATCCTTTACTGGCGGCCTTCATAGTTTTGTTCAACCCCCGATTGTTAGGCCCCACTTTTCCCATTATCACCCCATTTTG‑CTTGGACAGGTACCATTCCa                                                                                                              <  2:439982/133‑1 (MQ=255)
            aaCCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTcc                                                                                                               >  1:468201/1‑133 (MQ=255)
            aaCCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTcc                                                                                                               >  1:424861/1‑133 (MQ=255)
              cctacctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTAACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCAc                                                                                                             >  2:227648/1‑133 (MQ=255)
                 acctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGt                                                                                                          >  2:419890/1‑133 (MQ=255)
                 acctGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGt                                                                                                          >  1:381265/1‑133 (MQ=255)
                                       ggaggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAAc                                                                                    <  1:362310/133‑1 (MQ=255)
                                       ggaggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAAc                                                                                    <  1:387914/133‑1 (MQ=255)
                                       ggaggaTATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAAc                                                                                    <  2:465273/133‑1 (MQ=255)
                                              atTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCAGCGTATTAGGAATCCCGGTCTCGAACGTTTCCt                                                                             >  2:157085/1‑133 (MQ=255)
                                                  aTTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTgg                                                                         >  1:289505/1‑133 (MQ=255)
                                                     aCTGGCGGTCCTCATGGTTCTGCTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGttt                                                                      <  1:157085/133‑1 (MQ=255)
                                                     aCTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGttt                                                                      <  1:385063/133‑1 (MQ=255)
                                                       tGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCtg                                                                            >  1:335712/1‑125 (MQ=255)
                                                       tGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCtg                                                                            <  2:335712/125‑1 (MQ=255)
                                                       tGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTcc                                                                    >  2:33872/1‑133 (MQ=255)
                                                         gcggcCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCAt                                                                  >  1:81833/1‑133 (MQ=255)
                                                         gcggcCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCAt                                                                  >  2:314781/1‑133 (MQ=255)
                                                            gcCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAATCGTGTCTTGTGGTTTCCATccc                                                               >  1:178642/1‑133 (MQ=255)
                                                            gcCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATccc                                                               >  1:279052/1‑133 (MQ=255)
                                                                  aTAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATgcg                                                         <  2:468201/133‑1 (MQ=255)
                                                                           ttCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTAGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAAtc                                                >  2:506291/1‑133 (MQ=255)
                                                                              aaTCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAAtcttc                                             >  1:104137/1‑133 (MQ=255)
                                                                                tCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTtctc                                           >  1:14736/1‑133 (MQ=255)
                                                                                   aCGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGc                                        <  2:424861/133‑1 (MQ=255)
                                                                                        tGATAGGCACCACTTATCACATTACCAACCCACTTTGCATTTGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCaa                                   <  2:130619/133‑1 (MQ=255)
                                                                                          aTAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCTATCTTCTCAACATCAata                                 >  1:209119/1‑133 (MQ=255)
                                                                                          aTAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGc                                        >  1:319400/1‑126 (MQ=255)
                                                                                          aTAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAata                                 >  1:366510/1‑133 (MQ=255)
                                                                                          aTAGGCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTCCCATCCCAATGCGCGACCAATCTTCTCAGc                                        <  2:319400/126‑1 (MQ=255)
                                                                                             ggCACCACTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagca                              <  2:289505/133‑1 (MQ=255)
                                                                                               caccacTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAatagcata                            >  1:388454/1‑133 (MQ=255)
                                                                                               caccacTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAAGGCGGGACGAATCTTCTCAGCATCAatagcata                            >  2:187526/1‑133 (MQ=255)
                                                                                                   acTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAgcgg                        <  1:314781/133‑1 (MQ=255)
                                                                                                    cTTTTCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAgcggc                       >  2:13507/1‑133 (MQ=255)
                                                                                                     ttttCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAgcggcg                      <  2:360094/133‑1 (MQ=255)
                                                                                                       ttCACATTATCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGAt                    >  2:470894/1‑133 (MQ=255)
                                                                                                               aTCAACCCATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCGGCATCAAGAGCATAGCGGCGATCGTGTccc            >  1:185421/1‑133 (MQ=255)
                                                                                                                    cccATTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACg       <  2:279052/133‑1 (MQ=255)
                                                                                                                       aTTTTGCATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATc    <  2:14736/133‑1 (MQ=255)
                                                                                                                         tttGCATTGGGCAGGCACCATTCCGCCGTTTTTCGAATCCCACTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAg  <  2:178642/133‑1 (MQ=255)
                                                                                                                             |                                                                                                                                
GCTGTAGTTCCCAACCTACCTGCCCTGTTTTGCCAAAAAGGAGGATATTCATTACTGGCGGCCCTCATAGTTCTGTTCAATCCACGATTGATAGGCACCACTTTTCACATTATCAACCCATTTTGTATTGGACAGGTACCATTCCACCGTTTTACGAATCCCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAG  >  NC_000913/2111925‑2112178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑