| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,195,930 | T→C | V544A (GTG→GCG) | metG → | methionine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,195,930 | 0 | T | C | 100.0% | 38.9 / NA | 13 | V544A (GTG→GCG) | metG | methionine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/4); total (9/4) | |||||||||||
CGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGTGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGCTAAAGTTGACCTGCGCGTGGCGCTGATTGA > NC_000913/2195803‑2196059 | cGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAg > 2:270861/1‑133 (MQ=255) aaCAGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGCGAATCCGTTCAAGGCGCTGTATAACAGCATCGATATGAGGCGGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGCAAAAGCCGc > 2:1668/1‑133 (MQ=255) aCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCcg < 1:270861/133‑1 (MQ=255) ggATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCCCTAAAGACGAAGTAAAAGCCGCTGCCGCGCCg > 2:216851/1‑133 (MQ=255) aTGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGt < 1:414617/133‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGAGCCGGTAAc > 2:356582/1‑133 (MQ=255) cAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGc > 1:66454/1‑133 (MQ=255) ccGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTgg > 1:347735/1‑133 (MQ=255) ggCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATc < 2:260893/133‑1 (MQ=255) ttCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGAAACTGGCCAGCTCGTACATGATCCGTTTCAGTAAACCATCa > 2:222536/1‑133 (MQ=255) aCCGCATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGc < 2:19928/133‑1 (MQ=255) cATCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGCTaaa > 2:46600/1‑133 (MQ=255) tCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGAACCGATTCATGAAGCCAGCACCTTTGGCGACTTCGCTAAAGt > 2:170770/1‑133 (MQ=255) tCGATATGAGGCAGGTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGACAGATGACCCGATTCACGAATCCAGAACCTTTGGCGACTCCGCTaaatt > 2:239990/1‑131 (MQ=255) gTTGAAGCACTGGCGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGCTAAAGTTGACCTGCGCGTgg > 1:372189/1‑133 (MQ=255) tggcggAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGCTAAAGTTGACCTGCGCGTGGCGCTGATTGa > 2:415957/1‑133 (MQ=255) | CGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCACTGGTGGAAGCCTCTAAAGAAGAAGTAAAAGCCGCTGCCGCGCCGGTAACTGGCCCGCTGGCAGATGATCCGATTCAGGAAACCATCACCTTTGACGACTTCGCTAAAGTTGACCTGCGCGTGGCGCTGATTGA > NC_000913/2195803‑2196059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |