Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,182	A→G	V410A (GTA→GCA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,182	0	A	G	100.0%	105.8 / NA	32	V410A (GTA→GCA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

ACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGC  >  NC_000913/2260055‑2260294                                                                                                                                |                                                                                                                 aCGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATCCCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGa                                                                                                             <  2:284934/133‑1 (MQ=255) aCGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGa                                                                                                             <  1:124873/133‑1 (MQ=255) aCGCGCCACCATTGTCGCTAGACCCATTGCAAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGa                                                                                                             <  1:142960/133‑1 (MQ=255)      ccaccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGcc                                                                                                        <  2:76226/133‑1 (MQ=255)      ccaccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGcc                                                                                                        <  1:263573/133‑1 (MQ=255)       caccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGccc                                                                                                       <  1:483922/133‑1 (MQ=255)           aTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGt                                                                                                   <  2:429139/133‑1 (MQ=255)                tgCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTa                                                                                              <  1:394112/133‑1 (MQ=255)                tgCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTa                                                                                              <  1:298332/133‑1 (MQ=255)                 gCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTac                                                                                             >  1:338714/1‑133 (MQ=255)                 gCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTac                                                                                             >  2:316131/1‑133 (MQ=255)                      aCCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACAcatc                                                                                        >  1:288756/1‑133 (MQ=255)                         cATTGCCAGCGGTGGCCCCATCCCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATg                                                                                     <  2:254631/133‑1 (MQ=255)                         cATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATg                                                                                     <  1:474706/133‑1 (MQ=255)                          aTTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGc                                                                                    >  1:273549/1‑133 (MQ=255)                          aTTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCCCCCTTGTCAGTACGCATCATGc                                                                                    >  1:203561/1‑133 (MQ=255)                                        cACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGcc                                                                      >  2:259387/1‑133 (MQ=255)                                         aCCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGccc                                                                     <  2:273549/133‑1 (MQ=255)                                         aCCATACCTGACGCCTTAATCGCCTCCATCGGCCCATAGGTGTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGccc                                                                     <  2:203561/133‑1 (MQ=255)                                            aTACCTGCCGCCGTAATCCCCGCCATCGGGCCATAGGGTTGAGTACTCAGCAGAACCACACCGAATGCGTACGCTGGTTTGTTTGCCGGACCGCCCATGTCAGGACACATCATGCCACGGAGGATCGCCCCca                                                                  >  2:323129/1‑133 (MQ=255)                                                       cATAATCGCCGCCATCGGGCCATAGGTTAGAGCACTCAGCAAACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCa                                                       <  2:183068/133‑1 (MQ=255)                                                        aTAATCGCCGCCATCGGGCCATAGATTTGAGTCCTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCAt                                                      <  2:182265/133‑1 (MQ=255)                                                            tCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGc                                                  <  2:338714/133‑1 (MQ=255)                                                              gccgccATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAg                                                <  2:288756/133‑1 (MQ=255)                                                                                gTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGcc                              <  2:49224/133‑1 (MQ=255)                                                                                         cTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCa                     >  2:98698/1‑133 (MQ=255)                                                                                          tCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAg                    >  1:368441/1‑133 (MQ=255)                                                                                            agcagACCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGcc                  >  1:336171/1‑133 (MQ=255)                                                                                              cagacccagaccGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCt                >  2:448346/1‑133 (MQ=255)                                                                                                 aCCCACACCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCg             >  1:127676/1‑133 (MQ=255)                                                                                                      cacCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAAt        >  1:371950/1‑133 (MQ=255)                                                                                                      cacCGAATGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTAAGTCAGCCCTTCGAGAAt        >  1:209640/1‑133 (MQ=255)                                                                                                           aaggCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAg   >  2:330777/4‑133 (MQ=255)                                                                                                            aTGCGTACGCTGCTTTGTTTGCCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGc  >  2:76370/1‑133 (MQ=255)                                                                                                                                |                                                                                                                 ACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGC  >  NC_000913/2260055‑2260294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑