Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,268,347	T→C	G173G (GGT→GGC)	yeiR →	zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,268,347	0	T	C	100.0%	61.7 / NA	20	G173G (GGT→GGC)	yeiR	zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

AGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAA  >  NC_000913/2268225‑2268470                                                                                                                           |                                                                                                                            aGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGAc                                                                                                                   <  1:408959/133‑1 (MQ=255) aGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGAc                                                                                                                   <  2:47739/133‑1 (MQ=255) aGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGAc                                                                                                                   <  1:73654/133‑1 (MQ=255)        gCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACaattaat                                                                                                            <  2:509927/133‑1 (MQ=255)         cAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACaattaatt                                                                                                           <  2:86423/133‑1 (MQ=255)                      cGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATg                                                                                              >  1:471575/1‑133 (MQ=255)                      cGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATg                                                                                              >  1:95111/1‑133 (MQ=255)                                    tgCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAACTAATTCAGAGTGAGCAGGGGAAAGTTGACGGt                                                                                >  2:179659/1‑133 (MQ=255)                                     gCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTc                                                                               <  2:471575/133‑1 (MQ=255)                                      ccGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCa                                                                              <  1:301072/133‑1 (MQ=255)                                                    gTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGc                                                                <  2:496568/133‑1 (MQ=255)                                                                   gACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAg                                                 >  1:71586/1‑133 (MQ=255)                                                                       gTATGACGCCCGAAAGCGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGCCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGa                                             >  2:69013/1‑133 (MQ=255)                                                                        tACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAg                                            >  1:404999/1‑133 (MQ=255)                                                                           gacgCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTg                                         <  1:69013/133‑1 (MQ=255)                                                                                       tGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgcc                             <  2:441800/133‑1 (MQ=255)                                                                                            aaGCGCTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                        <  2:117839/133‑1 (MQ=255)                                                                                              gcgcTACAGCGTTGGTGGCAGCAAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt                      <  2:404999/133‑1 (MQ=255)                                                                                                                  gcaAAATGGCGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGTGCCGCGCATTCTCAGCAGCATGTTGTGaaa  >  1:205103/1‑133 (MQ=255)                                                                                                                  gcaAAATGGCCGCGATCGACAATTGATTCACAGTGAGCATGTGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaa  <  2:205103/133‑1 (MQ=255)                                                                                                                           |                                                                                                                            AGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAA  >  NC_000913/2268225‑2268470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑