Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,309,422	A→G	D328D (GAT→GAC)	ada ←	DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,309,422	0	A	G	100.0%	70.6 / NA	21	D328D (GAT→GAC)	ada	DNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/13);  total (8/13)

GGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGAC  >  NC_000913/2309301‑2309544                                                                                                                          |                                                                                                                           ggCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGac                                                                                                                 <  2:356017/133‑1 (MQ=255)           aCGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGGCACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGa                                                                                                       <  1:399580/133‑1 (MQ=255)           aCGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGa                                                                                                       <  1:373180/133‑1 (MQ=255)           aCGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGa                                                                                                       <  1:442670/133‑1 (MQ=255)             ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACa                                                                                                     <  2:439804/133‑1 (MQ=255)             ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACa                                                                                                     <  2:365274/133‑1 (MQ=255)             ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACa                                                                                                     <  1:431517/133‑1 (MQ=255)              gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAg                                                                                                    <  2:449196/133‑1 (MQ=255)                        ggCAAACAGATCCAATATTACCACTCCTCAATTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTGCGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGa                                                                                          <  1:232283/133‑1 (MQ=255)                                 aTCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTGACCACGGATCACACCACGACAGGGTATTATAGTAGCCAgat                                                                                 >  2:232283/1‑131 (MQ=255)                                  tCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCtt                                                                                <  2:123848/133‑1 (MQ=255)                                           aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTggcggc                                                                       >  2:363312/1‑133 (MQ=255)                                                 cctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGgc                                                                 >  2:501428/1‑133 (MQ=255)                                                          cAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAAc                                                        >  1:17983/1‑133 (MQ=255)                                                          cAGCTTCGAGGCGCACCAGTTGCGCTTTACACGACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAAc                                                        <  1:236770/133‑1 (MQ=255)                                                                                          gACACGCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGt                        <  1:363312/133‑1 (MQ=255)                                                                                               gCCCCAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGCACCGCTTGCGGGTTTCCGATGGCGTTAGcc                   >  1:222577/1‑133 (MQ=255)                                                                                                 cccAGCGGTAACCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAg                 <  2:17983/133‑1 (MQ=255)                                                                                                           aCCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATaa       >  1:270055/1‑133 (MQ=255)                                                                                                           aCCGGAAAGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGCTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGATTGCCGAGGGCGTTAGCCAGTCGCTGATaa       >  1:174002/1‑133 (MQ=255)                                                                                                                aaaGTGTGCCGTCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGAc  >  1:430967/1‑133 (MQ=255)                                                                                                                          |                                                                                                                           GGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGAC  >  NC_000913/2309301‑2309544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑