Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,141	A→G	G203G (GGT→GGC)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,141	0	A	G	100.0%	107.6 / NA	32	G203G (GGT→GGC)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

ACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCG  >  NC_000913/2312015‑2312264                                                                                                                               |                                                                                                                            accaccAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCATCGATACCGAAACTTTCATAATCATAAGTGATAGAGccgccg                                                                                                                       >  2:190442/1‑133 (MQ=255) accaccAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGccgccg                                                                                                                       >  1:63576/1‑133 (MQ=255)   caccaGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGAc                                                                                                                     >  1:427063/1‑133 (MQ=255)     ccaGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGAcgc                                                                                                                   >  2:451673/1‑133 (MQ=255)               tttCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGtt                                                                                                         >  1:439930/1‑133 (MQ=255)                 tCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGAcgccgtcgc                                                                                                             <  2:72643/127‑1 (MQ=255)                 tCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGAcgccgtcgc                                                                                                             >  1:72643/1‑127 (MQ=255)                   aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGa                                                                                                     >  2:43599/1‑133 (MQ=255)                     cACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACg                                                                                                   <  1:116222/133‑1 (MQ=255)                     cACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACg                                                                                                   <  2:48709/133‑1 (MQ=255)                     cACGGTCGCCATTACCGATGTAAGCAGCAGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACg                                                                                                   <  2:131306/133‑1 (MQ=255)                        ggTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGCGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCGGCCGACGCCGTCGCCGTTTTGACGCAg                                                                                                >  2:46416/1‑133 (MQ=255)                         gTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGt                                                                                               <  2:63576/133‑1 (MQ=255)                                   ccGATGTAGGCAGCGTTGATCTCAGCATCACTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGAc                                                                                     <  1:190442/133‑1 (MQ=255)                                           agcagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACCCAGCGCGTCACGATCGTTgtta                                                                             >  1:216124/1‑133 (MQ=255)                                             cagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttagt                                                                           >  1:108304/1‑133 (MQ=255)                                                  gtgtTCTGAGTATCAGTACGTTTGGAGCTGGACATCGCACCCCCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGc                                                                      <  2:216124/133‑1 (MQ=255)                                                    gtTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCa                                                                    <  2:439930/133‑1 (MQ=255)                                                          aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTa                                                              <  1:415922/133‑1 (MQ=255)                                                              tCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGc                                                          <  1:451673/133‑1 (MQ=255)                                                               cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAg                                                           >  2:478641/1‑131 (MQ=255)                                                               cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAg                                                           <  1:478641/131‑1 (MQ=255)                                                               cAGTACGTTTGGAGCTGGAGATCGCACCA‑CGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCAGGACGGTTATTAGTTACGCCACGAGTAAAGCCt                                                        >  2:192045/1‑133 (MQ=255)                                                                                           accGATTCCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                             <  2:427063/133‑1 (MQ=255)                                                                                           accGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                             <  1:43599/133‑1 (MQ=255)                                                                                           accGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                             <  2:68922/133‑1 (MQ=255)                                                                                            ccGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCCCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCt                            >  2:512512/1‑133 (MQ=255)                                                                                               aTACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                         >  1:355403/1‑133 (MQ=255)                                                                                                tACCGAAACTTTCGTTATCTTAAGTGATAGAGCCGCCGACGCCGTCGCTGTTTTGTCGCAGTGCGTCACGACCGTTGTTAGTTACGTCACTAGTAAAGCCTTCACCAGATTGGTTGCCGTTTTTACCCTGGTa                        <  2:108304/133‑1 (MQ=255)                                                                                                 aCCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGAGGGGTTGCCGTTTTTACCCTGGTAc                       >  2:215144/1‑133 (MQ=255)                                                                                                          ttCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa              >  2:113699/1‑133 (MQ=255)                                                                                                           tCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaa             >  1:408773/1‑133 (MQ=255)                                                                                                                 tCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa       <  1:46416/133‑1 (MQ=255)                                                                                                                      aGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCg  >  2:409883/1‑133 (MQ=255)                                                                                                                               |                                                                                                                            ACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCG  >  NC_000913/2312015‑2312264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑