Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,325,859	A→G	G368G (GGA→GGG)	atoE →	short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,325,859	0	A	G	100.0%	58.2 / NA	18	G368G (GGA→GGG)	atoE	short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

ACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAA  >  NC_000913/2325736‑2325983                                                                                                                            |                                                                                                                             aCTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTg                                                                                                                     >  2:56986/1‑133 (MQ=255)      ttACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATAcc                                                                                                                <  2:448951/133‑1 (MQ=255)         ccGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGc                                                                                                             <  1:56986/133‑1 (MQ=255)                             gCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCTAggcgcggggcgctg                                                                                         >  1:134729/1‑133 (MQ=255)                             gCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAggcgctgggcgctg                                                                                         >  1:340628/1‑133 (MQ=255)                                aaCAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGAtc                                                                                      >  2:8577/1‑133 (MQ=255)                                                    aaTGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCg                                                                  <  2:289274/133‑1 (MQ=255)                                                         ccTTTTTTAGTTCTGCACCGATCAACTTCGGCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGc                                                             <  1:194522/133‑1 (MQ=255)                                                              tttAGTGCGGCGCTGATTAACTTCGACGTTCCGGCTGGCGGCTGCCGATGTGTTACTCAGCGGCCTATGGTGATTCCCGCAGCTCAGGCGGTGGGCGCTAAACTCGGTAAGGCGGTAATGGCGATCGCCTACg                                                        <  2:68975/133‑1 (MQ=255)                                                                   ttCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAg                                                   >  1:484289/1‑133 (MQ=255)                                                                    tCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGc                                                  <  2:340628/133‑1 (MQ=255)                                                                    tCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGc                                                  <  2:484289/133‑1 (MQ=255)                                                                           tGATTAACTTCGCCGTTCCGTCTGACGGTGGTCACTGGGTTCTTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGAt                                           <  2:134729/133‑1 (MQ=255)                                                                             aTTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGTTAAATCGGGAATTGCGTTCGCCTACGGCGAACAATGGATGa                                         >  2:133605/1‑133 (MQ=255)                                                                              ttAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGaa                                        <  2:322848/133‑1 (MQ=255)                                                                                       ccGGTCCGACTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCAGATCTGCGTAAATCGGTAATGGCGATCCCCTACGGCGAGCAATGGATGAATATGGcaca                               >  2:308505/1‑133 (MQ=255)                                                                                               tCTGGCGGCGGTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCt                       >  1:101906/1‑133 (MQ=255)                                                                                                    cggcggTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCACTGGATGAACATCGCTCAACCATTCTGGgcg                  >  1:207910/1‑133 (MQ=255)                                                                                                         gTCACTGGGTTATTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGcc             >  1:393327/1‑133 (MQ=255)                                                                                                                    aTTCAGGGGCCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCaa  <  1:8577/133‑1 (MQ=255)                                                                                                                            |                                                                                                                             ACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCGGTAATGACCTTTTTTAGTTCTGCACTGATTAACTTCGCCGTTCCGTCTGGCGGCGGTCACTGGGTTATTCAGGGACCTTTCGTGATACCCGCAGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAA  >  NC_000913/2325736‑2325983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑