Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,402,849	A→G	F333L (TTC→CTC)	nuoC ←	NADH:quinone oxidoreductase subunit CD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,402,849	0	A	G	100.0%	71.5 / NA	22	F333L (TTC→CTC)	nuoC	NADH:quinone oxidoreductase subunit CD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

CCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAACACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGTTAACGCGATCCGGCACGGTGATCCCGGCCA  >  NC_000913/2402729‑2402977                                                                                                                         |                                                                                                                                 cccAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCAt                                                                                                                      >  1:391917/1‑133 (MQ=255)      ccGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTgcgc                                                                                                                 >  1:401495/1‑133 (MQ=255)      ccGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTgcgc                                                                                                                 >  1:81912/1‑133 (MQ=255)          gcgGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGAc                                                                                                             >  1:388307/1‑133 (MQ=255)          gcgGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGAc                                                                                                             <  2:285627/133‑1 (MQ=255)                    gTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATa                                                                                                   <  2:86725/133‑1 (MQ=255)                        gcTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAgg                                                                                               <  2:401495/133‑1 (MQ=255)                        gcTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAgg                                                                                               <  2:391917/133‑1 (MQ=255)                         cTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGt                                                                                              <  1:128473/133‑1 (MQ=255)                              ccgccAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCgaga                                                                                         <  2:120334/133‑1 (MQ=255)                              ccgccAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCgaga                                                                                         <  2:329027/133‑1 (MQ=255)                                               cGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTg                                                                        >  2:454014/1‑133 (MQ=255)                                                gCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGt                                                                       <  2:388307/133‑1 (MQ=255)                                                     gTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATg                                                                  <  1:46431/133‑1 (MQ=255)                                                               aaaCCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGAGATAAAGCAGGTGACTGGTGATGCGGAACAGtt                                                        >  2:209569/1‑133 (MQ=255)                                                                 aCCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACAATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCg                                                      <  1:439366/133‑1 (MQ=255)                                                                      tGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGagag                                                 >  1:353543/1‑133 (MQ=255)                                                                             ttCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAAcg                                          >  2:438620/1‑133 (MQ=255)                                                                                caccaGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGa                                       >  1:119344/1‑133 (MQ=255)                                                                                                    gACGATCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGTTAACGCGATCCgg                   <  2:119344/133‑1 (MQ=255)                                                                                                         tCGGTAAAGGCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGTTAACGCGATCCGGCACgg              <  1:457530/133‑1 (MQ=255)                                                                                                                  gCGAAGAGCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGTTAACGCGATCCGGCACGGTGATCCCgg     <  2:81912/133‑1 (MQ=255)                                                                                                                     aagagCACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGGTAACGCGATCCAGCTCGGTGAACCCGGCCa  >  1:249899/1‑133 (MQ=255)                                                                                                                         |                                                                                                                                 CCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAAGGCGAAGAACACTGGCGTCATTGCGCCGACGTCCTGAATAAAGGTCGAGATATACAGCAGGTGACTGTTGATGCGGAACAGTTCGGAGAGCATAACGCGAATGACGTTAACGCGATCCGGCACGGTGATCCCGGCCA  >  NC_000913/2402729‑2402977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑