Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,408,429 T→C L290P (CTA→CCA)  alaA → glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,408,4290TC93.3% 88.0 / ‑4.2 30L290P (CTA→CCA) alaAglutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (11/17);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGGGTGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCG  >  NC_000913/2408304‑2408553
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tCAGGGGTGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCCCAACACGTCATACAGACCGCGACAggtggt                                                                                                                       >  1:199544/1‑133 (MQ=255)
       tGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCaga                                                                                                                >  1:16648/1‑133 (MQ=255)
        ggATGGTGTTGAACGGGCCGAAAACACACGCCTAAGGCTACCTCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCagag                                                                                                               <  2:199544/133‑1 (MQ=255)
        ggATGGTGTTGAACGGGCCGAAAAAACCCGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCagag                                                                                                               <  1:353605/133‑1 (MQ=255)
         gATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGc                                                                                                              >  2:71631/1‑133 (MQ=255)
            ggTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCACCAGGTGGTTATCAGAGCATc                                                                                                           <  1:382247/133‑1 (MQ=255)
                 ttaaCGGGCCGAAAACACACGCCATAGGCTACTTCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGa                                                                                                      <  2:245800/131‑1 (MQ=255)
                 tGAACGGGCCGAAAATACACGCCATAGGCTACTTCGAAGGTATGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGa                                                                                                      <  2:188842/133‑1 (MQ=255)
                        gCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTGTCACAGCATCAGACAATttatt                                                                                               >  1:245800/1‑133 (MQ=255)
                        gCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTGTCAAAGGATCAGTGAATttatt                                                                                               >  1:149211/1‑133 (MQ=255)
                        gCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATttatt                                                                                               >  2:476173/1‑133 (MQ=255)
                        gCCGAAAAAACACGCCAAAGGCTACATCGAAGGGCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGTTAGTTGGATATCAGAGGATCAATGAATttatt                                                                                               >  2:167327/1‑133 (MQ=255)
                                          aGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTAATCAGAGCATCAATAACTTTATTACGCCTGGAGgtcgtcct                                                                             >  2:232474/1‑131 (MQ=255)
                                                   cGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGagcag                                                                    <  1:343202/133‑1 (MQ=255)
                                                     aaGGTCTGGAAATGCTGTCTTCAATGCGCCTGTGTGCTAACGATGCTCCGCAACACGCCATTCAGACCGTGTCAGGTGCTTATCAGTGCCTTAGTGAATTAATTCCCCATGGCGGTCAGCTTCATAAgcggcg                                                                  >  2:203523/1‑133 (MQ=255)
                                                      aGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGATATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGt                                                                 >  2:110299/1‑133 (MQ=255)
                                                        gTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa                                                               <  1:114033/133‑1 (MQ=255)
                                                           tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACcg                                                            <  2:445234/133‑1 (MQ=255)
                                                                  gCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTgg                                                     >  2:6907/1‑133 (MQ=255)
                                                                  gCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTgg                                                     >  1:6133/1‑133 (MQ=255)
                                                                     ggCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGaa                                                  <  2:6133/133‑1 (MQ=255)
                                                                          cAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGAt                                             <  1:475198/133‑1 (MQ=255)
                                                                          cAATGCGCATGTGTGATACCGTCCCTGCGCTACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGAt                                             <  1:167327/133‑1 (MQ=255)
                                                                                                 ccTGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCt                      >  1:406283/1‑133 (MQ=255)
                                                                                                   tGCGCAACACGCCATTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGc                    <  2:16648/133‑1 (MQ=255)
                                                                                                                aTTCAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCgtg       <  1:71631/133‑1 (MQ=255)
                                                                                                                   cAGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCgtggtg    <  1:476173/133‑1 (MQ=255)
                                                                                                                    agtccGCGCCAGGTGGTTTTCAGAGCATCATTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTgc   <  1:232474/130‑1 (MQ=255)
                                                                                                                    aGACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCTGGGCGTTACCTTCGAGAAACCCCGTGGTgc   >  1:145983/1‑133 (MQ=255)
                                                                                                                     gACCGCGCCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTgcg  <  2:406283/133‑1 (MQ=255)
                                                                                                                     gACCGCGCCACGTGGCTATCAGAGTATCTGTGCATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTgcg  <  2:145983/133‑1 (MQ=255)
                                                                                                                         gcgcCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCgtggtg    <  2:310508/127‑1 (MQ=255)
                                                                                                                         gcgcCAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCgtggtg    >  1:310508/1‑127 (MQ=255)
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TCAGGGGTGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCG  >  NC_000913/2408304‑2408553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑