Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,418,281	G→A	A401T (GCC→ACC)	yfcC →	putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,418,281	0	G	A	100.0%	85.4 / NA	28	A401T (GCC→ACC)	yfcC	putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/11);  total (17/11)

CTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTAC  >  NC_000913/2418155‑2418408                                                                                                                               |                                                                                                                                ctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTTGACGACACGGTCGGGACCTGGt                                                                                                                           >  1:132809/1‑133 (MQ=255) ctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGt                                                                                                                           <  1:352579/133‑1 (MQ=255) ctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGt                                                                                                                           <  1:511879/133‑1 (MQ=255)         ttttgCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTa                                                                                                                        >  1:267835/5‑128 (MQ=255)         ttttGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTa                                                                                                                        <  2:267835/128‑1 (MQ=255)                   gggATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAgg                                                                                                         <  2:86979/133‑1 (MQ=255)                                                 gCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGtt                                                                           >  2:273258/1‑133 (MQ=255)                                                 gCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGtt                                                                           >  1:30280/1‑133 (MQ=255)                                                         tGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAg                                                                   <  2:54560/133‑1 (MQ=255)                                                           ccAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTGCGTGACGTCCGGTTCTGGTCAggc                                                                 >  2:218379/1‑133 (MQ=255)                                                                          ccATCCTCAAAAGCACTGCCAATGTCATGAGCTGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGcc                                                  <  2:132809/133‑1 (MQ=255)                                                                           cATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCg                                                 >  2:475652/1‑133 (MQ=255)                                                                                    cAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCa                                        <  2:279413/133‑1 (MQ=255)                                                                                    cAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCa                                        >  2:501422/1‑133 (MQ=255)                                                                                       cATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCg                                     >  2:379854/1‑133 (MQ=255)                                                                                         ttGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCt                                   >  2:433448/1‑133 (MQ=255)                                                                                         ttGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCAGGATCTGGTCAGGCGGCGTTAACCATGCCGTTAATGGCACCGCt                                   >  2:110656/1‑133 (MQ=255)                                                                                          tGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGGGTTAACCATGCCGTTACTGGCACCGCtt                                  >  2:150480/1‑133 (MQ=255)                                                                                          tGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCtt                                  >  1:426823/1‑133 (MQ=255)                                                                                          tGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCtt                                  >  1:41928/1‑133 (MQ=255)                                                                                                tGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGAt                            <  2:30280/133‑1 (MQ=255)                                                                                                tGCCATTAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGAt                            >  2:449381/1‑133 (MQ=255)                                                                                                     ttAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGt                       <  1:501422/133‑1 (MQ=255)                                                                                                      tAGCGGTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTc                      <  1:449381/133‑1 (MQ=255)                                                                                                           gTCTGGACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGgtgt                 >  2:284795/1‑133 (MQ=255)                                                                                                                gACAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAAcc            >  2:360291/1‑133 (MQ=255)                                                                                                                 aCAACGCGGTCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCg           <  1:475652/133‑1 (MQ=255)                                                                                                                          tCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTAc  >  1:27828/1‑133 (MQ=255)                                                                                                                          tCGCGACCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGATGATAACCGACAGGTTAc  >  1:196425/1‑133 (MQ=255)                                                                                                                               |                                                                                                                                CTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTAC  >  NC_000913/2418155‑2418408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑