Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,425,237	T→C	D227G (GAC→GGC)	hisQ ←	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,425,237	0	T	C	100.0%	52.4 / NA	16	D227G (GAC→GGC)	hisQ	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/12);  total (4/12)

GCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAC  >  NC_000913/2425110‑2425360                                                                                                                                |                                                                                                                            gcgcCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGcc                                                                                                                        <  1:16672/133‑1 (MQ=255)  cgcCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGccc                                                                                                                       >  2:439400/1‑133 (MQ=255)   gcCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCct                                                                                                                      >  1:410071/1‑133 (MQ=255)                        aCCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAgcg                                                                                                 <  2:425393/133‑1 (MQ=255)                            ccAAAATAAGCAGGCACAGAGTGATCGCCACCCCAGTAAAGCGATAACCATCGGAGCACATCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGACCAGCCCTCTTCACACCCACAGAGTAGCGacgc                                                                                             >  2:106571/2‑133 (MQ=255)                                    agcagcCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGaa                                                                                     <  1:334251/133‑1 (MQ=255)                                         ccACAGAGCGATCGCCACCCCATTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAAcagca                                                                                <  1:159174/133‑1 (MQ=255)                                            caGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAgcagca                                                                             <  1:294721/133‑1 (MQ=255)                                            caGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAgcagca                                                                             <  2:410071/133‑1 (MQ=255)                                            caGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAgcagca                                                                             <  1:80380/133‑1 (MQ=255)                                              gagTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGcaca                                                                           <  1:508690/133‑1 (MQ=255)                                                gTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACAcc                                                                         <  1:301603/133‑1 (MQ=255)                                                               gTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAgtggt                                                          >  2:461417/1‑133 (MQ=255)                                                                              tCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAAt                                           <  2:46905/133‑1 (MQ=255)                                                                                            cGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGa                             <  1:461417/133‑1 (MQ=255)                                                                                                                 gATTTCGATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTccc        <  1:439400/133‑1 (MQ=255)                                                                                                                       gATCACAGGCCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAAGCACGCCACAGACAATGGCGAAATAGAACGGATCCCAGGTAc  >  2:133880/1‑133 (MQ=255)                                                                                                                                |                                                                                                                            GCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAC  >  NC_000913/2425110‑2425360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑