Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,440,162	T→C	intergenic (‑42/+223)	yfcJ ← / ← fabB	putative transporter YfcJ/beta‑ketoacyl‑[acyl carrier protein] synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,440,162	0	T	C	100.0%	52.1 / NA	17	intergenic (‑42/+223)	yfcJ/fabB	putative transporter YfcJ/beta‑ketoacyl‑[acyl carrier protein] synthase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/4);  total (13/4)

GAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGG  >  NC_000913/2440043‑2440290                                                                                                                        |                                                                                                                                 gagaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAAGCGAGGAGTATCCCCGCCTCAGCTCTCTgg                                                                                                                     >  1:233411/1‑133 (MQ=255)   gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGtt                                                                                                                   >  1:468280/1‑133 (MQ=255)         ccGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAggg                                                                                                             >  2:427745/1‑133 (MQ=255)                               cGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGAttt                                                                                       >  1:64844/1‑133 (MQ=255)                                                         cGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGaaa                                                             >  2:383335/1‑133 (MQ=255)                                                              gtgCTTACACTAGTCTTGCATTACTCCAAAAGTCAGCGAAACGGGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGAc                                                        <  2:233411/131‑1 (MQ=255)                                                                           tCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGtt                                           >  1:338401/1‑133 (MQ=255)                                                                                           aaTGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTggcgg                           <  1:427745/133‑1 (MQ=255)                                                                                             tGCAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGTCAAAGCGGGAAGTTTGCCTGGGACTGgcggcg                         >  2:188574/1‑133 (MQ=255)                                                                                               cAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGAt                       >  1:92096/1‑133 (MQ=255)                                                                                               cAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGAt                       >  2:9210/1‑133 (MQ=255)                                                                                               cAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGAt                       >  2:385830/1‑133 (MQ=255)                                                                                               cAGCGCAAGGCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAACTTTGCCGGGAACTGGCGGCGAt                       >  1:152950/1‑133 (MQ=255)                                                                                                       ggCGAGGAGTATCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAatg               >  1:181180/1‑133 (MQ=255)                                                                                                                 aTCCCCGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGaaaaa     >  1:489139/1‑133 (MQ=255)                                                                                                                   ccccGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAgg   <  2:64844/133‑1 (MQ=255)                                                                                                                    cccGCCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAggg  <  2:92096/133‑1 (MQ=255)                                                                                                                        |                                                                                                                                 GAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGG  >  NC_000913/2440043‑2440290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑