Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	43.5 / NA	15	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/5);  total (10/5)

TAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTG  >  NC_000913/2446454‑2446702                                                                                                                              |                                                                                                                            tAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCTGGCACGGCAATGCTg                                                                                                                      >  1:248242/1‑133 (MQ=255)       gATCGCCAGCATCGCTTCTGCGATCGGTACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATTGTACGACGCGGCACGGCAATGGTGGAGGTc                                                                                                                >  1:152724/1‑133 (MQ=255)              accATCGCTTCTGCGATCGGCACCCCGCAGATCCCGACACATGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCa                                                                                                         <  2:248242/131‑1 (MQ=255)                       tCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCatat                                                                                                >  2:325428/1‑133 (MQ=255)                                      gcgcGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgct                                                                                 <  1:325428/133‑1 (MQ=255)                                        gcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctgc                                                                               >  1:60471/1‑133 (MQ=255)                                                    acaGGTATGGTGACGGCCTTTGGTGATCATCTCAACTTTTTCGCCAAAGCGGTTAATGGTACGACCCGGCCCGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATa                                                                   <  2:60471/133‑1 (MQ=255)                                                    acaGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATa                                                                   <  2:427235/133‑1 (MQ=255)                                                        ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgc                                                               >  1:373501/1‑133 (MQ=255)                                                                            gATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                           <  2:373501/133‑1 (MQ=255)                                                                              tcatcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGtt                                         >  2:455449/1‑133 (MQ=255)                                                                                atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGc                                       >  2:417115/1‑133 (MQ=255)                                                                                           ttcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCg                            >  1:75806/1‑133 (MQ=255)                                                                                           ttcGCCAAAGCGGATAATGGGACGACCCGCCACGGCAATGCTGGAGGTCGGCTTCAGCGACATATGGGCAATGATTTGCTGCCCGCTGATGATACCGCAGAGAATGCTGCCCGCATGGTTGCTCTGTAAAACg                            >  2:428801/1‑133 (MQ=255)                                                                                                                 cGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGt      >  2:90348/1‑133 (MQ=255)                                                                                                                    cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGA‑TTCATCGCGGTTCTg  >  1:319949/1‑133 (MQ=255)                                                                                                                              |                                                                                                                            TAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTG  >  NC_000913/2446454‑2446702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑