Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,500,707	A→G	T109A (ACC→GCC)	ypdB →	DNA‑binding transcriptional activator YpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,500,707	0	A	G	100.0%	36.2 / NA	12	T109A (ACC→GCC)	ypdB	DNA‑binding transcriptional activator YpdB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

CCATAAACCGTTTATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTACCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGACACCATTAATCTGGTGAAAGATGAGCGAA  >  NC_000913/2500598‑2500821                                                                                                              |                                                                                                                   ccATAAACCGTTTATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGaa                                                                                             <  1:352107/133‑1 (MQ=255)      aaCCGTTTATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGc                                                                                        <  2:491174/133‑1 (MQ=255)           tttATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATTAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGaa                                                                                             >  1:481079/1‑123 (MQ=255)           tttATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATTAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGaa                                                                                             <  2:481079/123‑1 (MQ=255)           tttATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGGGGCCTGGc                                                                                   >  1:174395/1‑133 (MQ=255)                   gtTCACCACCCCGTGTAAAGAACATGCGGGAGTATCGTGTGTACTGGTGGCGTTTGGCTACAATCCCAAACCGTATCAGGAGGCACGTATTGCCGGGATGCTGCAAAGACTGGAAGCGGCCTGGCAACAAcag                                                                           <  2:134009/133‑1 (MQ=255)                         caCCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGAcc                                                                     >  1:50324/1‑133 (MQ=255)                                tGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCTAAAACTGGTAGCGGCCGGGCAACAATAGCAGACCAGCAGTa                                                              >  2:220282/1‑133 (MQ=255)                                                       gTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAAcgctt                                       <  2:50324/133‑3 (MQ=255)                                                        tttGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTg                                      >  1:514864/1‑133 (MQ=255)                                                                ggAGGCGTTTGACTACATTCTCAACCCGAATCAGGAGTCACTTATTGCCGGGATGCAGCAAAAACTGGAAGCGGCCTGGAAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGac                              <  2:150615/133‑1 (MQ=255)                                                                      gTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGACACCAtt                        >  1:113477/1‑133 (MQ=255)                                                                         tGACTACATTCTCAAACCGTATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGCCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGACACCATTAAt                     <  2:514864/133‑1 (MQ=255)                                                                                            tATCAGGAGTCACGTATTGCCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGACACCATTAATCTGGTGAAAGATGAGCGaa  >  2:85141/1‑133 (MQ=255)                                                                                                              |                                                                                                                   CCATAAACCGTTTATTGTGTTCATCACCGCGTGGAAAGAACATGCGGTAGAAGCGTTTGAACTGGAGGCGTTTGACTACATTCTCAAACCGTATCAGGAGTCACGTATTACCGGGATGCTGCAAAAACTGGAAGCGGCCTGGCAACAACAGCAGACCAGCAGTACGCCTGCCGCGACGGTAACGCGTGAGAATGACACCATTAATCTGGTGAAAGATGAGCGAA  >  NC_000913/2500598‑2500821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑