Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,575,328	T→C	T44A (ACG→GCG)	eutP ←	putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,575,328	0	T	C	100.0%	71.6 / NA	23	T44A (ACG→GCG)	eutP	putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

AGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTT  >  NC_000913/2575201‑2575453                                                                                                                                |                                                                                                                              aGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCaa                                                                                                                          >  2:305248/1‑133 (MQ=255)         ggcaggcaACCGACTTTCCGGGACATTTGCACCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGccc                                                                                                                  <  1:157349/133‑1 (MQ=255)          gcaggcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCt                                                                                                                 >  1:411937/1‑133 (MQ=255)                           cGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACg                                                                                                <  2:267469/133‑1 (MQ=255)                                           tGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTgg                                                                                >  2:21807/1‑133 (MQ=255)                                            ggACATAGCTCAGCAGGTTTACATCCTGTACCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGc                                                                               <  1:205493/133‑1 (MQ=255)                                                      cAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATaa                                                                     <  1:21807/133‑1 (MQ=255)                                                       aGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAAt                                                                    >  2:284909/1‑133 (MQ=255)                                                                aCATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCa                                                           <  1:284909/133‑1 (MQ=255)                                                                      tGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAt                                                     >  1:288372/1‑133 (MQ=255)                                                                      tGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAt                                                     >  2:68204/1‑133 (MQ=255)                                                                       gTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAtt                                                    >  2:68662/1‑133 (MQ=255)                                                                       gTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAtt                                                    >  1:111534/1‑133 (MQ=255)                                                                       gTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAtt                                                    >  2:39249/1‑133 (MQ=255)                                                                       gTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAtt                                                    >  2:327357/1‑133 (MQ=255)                                                                         aGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTaa                                                  >  2:492500/1‑133 (MQ=255)                                                                          gCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTaaa                                                 <  1:327357/133‑1 (MQ=255)                                                                           cGTGGAAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCCCCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAAt                                                <  2:320805/133‑1 (MQ=255)                                                                            gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGATTTTCTGGCGAGAGTAAAATTCCCCTGCAACGCATTAAATa                                               >  2:176072/1‑133 (MQ=255)                                                                                        gCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGCTTTTc                                   >  2:50568/1‑133 (MQ=255)                                                                                                           ggTTAAAATATTCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCcac                <  1:496853/133‑1 (MQ=255)                                                                                                                     ttCACCCGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAAtt      <  1:305248/133‑1 (MQ=255)                                                                                                                         cccGGCGCATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGtt  <  2:111534/133‑1 (MQ=255)                                                                                                                                |                                                                                                                              AGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTT  >  NC_000913/2575201‑2575453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑