Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,605,744	T→C	F312L (TTC→CTC)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,605,744	0	T	C	100.0%	92.8 / NA	28	F312L (TTC→CTC)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/13);  total (15/13)

GCATGTGATTGGCGGCGTTGGCATGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCT  >  NC_000913/2605614‑2605870                                                                                                                                   |                                                                                                                               gCATGTGATTGGCGGCGTTGGCATGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGctc                                                                                                                              >  1:102150/1‑133 (MQ=255)         ttGGCGGCGTTGGCATGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCtt                                                                                                                      <  1:115377/133‑1 (MQ=255)           ggcggcGTTGGCATGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCTCTCAACTTGGCTGGATGCCCTTCGGCTTGt                                                                                                                    <  2:94332/133‑1 (MQ=255)                        tGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCtt                                                                                                       <  2:274798/133‑1 (MQ=255)                               ggCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTAGTCCCTGTCCATCTTCGGCTcg                                                                                                >  1:163424/1‑133 (MQ=255)                                   cTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGc                                                                                            <  1:88363/133‑1 (MQ=255)                                       tCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGgc                                                                                        >  2:472430/1‑133 (MQ=255)                                         aCCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCGCTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGgcgc                                                                                      >  2:508983/1‑133 (MQ=255)                                         aCCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCACTTCGGCTTGTCGCTCACCATCTTCGGCTCACGGCTGgcgc                                                                                      >  2:144000/1‑133 (MQ=255)                                                 ttATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCAATCTTCGGCTCACGACTGGCGTTGGAGGgc                                                                              >  1:186698/1‑133 (MQ=255)                                                    tGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagc                                                                           <  1:366480/133‑1 (MQ=255)                                                    tGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagc                                                                           <  1:508983/133‑1 (MQ=255)                                                     ggCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagca                                                                          <  2:56021/133‑1 (MQ=255)                                                     ggCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagca                                                                          >  1:44964/1‑133 (MQ=255)                                                     ggCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagca                                                                          >  1:506216/1‑133 (MQ=255)                                                     ggCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGgcagca                                                                          >  1:56021/1‑133 (MQ=255)                                                             gATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTAc                                                                  <  2:102150/133‑1 (MQ=255)                                                                            gcagGATATGGAGCGGTTGCTAGCGTGGTCGATCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCAcgcg                                                   <  2:186698/133‑1 (MQ=255)                                                                              agGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGtt                                                 <  2:394751/133‑1 (MQ=255)                                                                                          ggTTGCTAGCTTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCt                                     >  2:261564/1‑133 (MQ=255)                                                                                          ggTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCt                                     >  1:474050/1‑133 (MQ=255)                                                                                           gTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTg                                    >  2:445683/1‑133 (MQ=255)                                                                                            ttGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATGGTCAACCCCCCGCTCGCCAAAAGCCTGt                                   >  2:202086/1‑133 (MQ=255)                                                                                            ttGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGt                                   >  2:446355/1‑133 (MQ=255)                                                                                             tGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGtt                                  <  2:420564/133‑1 (MQ=255)                                                                                              gCTAGCTTGGTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGttt                                 <  1:261564/133‑1 (MQ=255)                                                                                                      ggTCGACCATCACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGt                         >  2:292828/1‑133 (MQ=255)                                                                                                                cACTCAACTTGGCTGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTgcg               <  2:506216/133‑1 (MQ=255)                                                                                                                cACTCAACCTGGCTGTATCCTCCTCGGCTTCTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTgcg               <  1:144000/133‑1 (MQ=255)                                                                                                                             tGGATGCTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCt  >  1:459913/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                               GCATGTGATTGGCGGCGTTGGCATGGTCATGGCACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGCAGGATATGAAGCGGTTGCTAGCCTGGTCGACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCT  >  NC_000913/2605614‑2605870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑