| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,658,436 | C→T | G167D (GGC→GAC) | hscA ← | iron‑sulfur cluster biosynthesis chaperone HscA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,658,436 | 0 | C | T | 90.9% | 26.6 / NA | 11 | G167D (GGC→GAC) | hscA | iron‑sulfur cluster biosynthesis chaperone HscA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/5); minor base G (1/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGC > NC_000913/2658316‑2658545 | tCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCACAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTggg < 2:43099/133‑1 (MQ=255) cATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACTCGCCGCGTCTTGGGTGTCCTGACGCTGGGc > 1:253548/1‑133 (MQ=255) tAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCGCGCCAGACTCGCAGCGACTGTGGTGGCCTGGCGCTGGGCAt > 2:132033/1‑133 (MQ=255) tCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGaa < 2:61946/133‑1 (MQ=255) gACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt > 2:288196/1‑133 (MQ=255) cGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGAt < 1:288196/133‑1 (MQ=255) aGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAATACACCGCAACGGTGACAACTGCACCATCCAg > 2:169520/1‑133 (MQ=255) gggcAGGACGCGAAGCCCCGCCATTCGCACCGCGTCTTTGGAGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCgccgcc < 2:253548/131‑1 (MQ=255) cgcAGGACGTGAAGCCCCGCCAGACGCGCCGCGGCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGGTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCa < 2:386728/133‑1 (MQ=255) ccGCCAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGATGCCCGCGCCGACAATGCTTTGAGGATGTc > 1:183280/1‑133 (MQ=255) ccAGACGCGCCGCGTCTTTGGTGTCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGCGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGATTTGAGGATGGCCGc > 1:125763/1‑133 (MQ=255) | TCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGC > NC_000913/2658316‑2658545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |