Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,669,362	T→C	S111P (TCT→CCT)	hcaE →	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,669,362	0	T	C	100.0%	88.0 / NA	28	S111P (TCT→CCT)	hcaE	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/16);  total (12/16)

TCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAG  >  NC_000913/2669247‑2669479                                                                                                                    |                                                                                                                      tCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGc                                                                                                      >  1:61858/1‑133 (MQ=255) tCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGc                                                                                                      >  1:382550/1‑133 (MQ=255)  cAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCg                                                                                                     <  2:267609/133‑1 (MQ=255)      aaGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGtt                                                                                                 <  1:501697/133‑1 (MQ=255)      aaGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGtt                                                                                                 <  2:438092/133‑1 (MQ=255)         aCGGCAGCCTCAAGGTGTTTCTAAACCAATACCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGAt                                                                                              <  1:203557/133‑1 (MQ=255)                          tttCTCAACCAATGCCGCCACCGGGCCATGCGCGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGCTGATCGATGTACCGCTGGAAc                                                                             >  2:416905/1‑133 (MQ=255)                                  ccAATGCCGGCACGGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAAGGCTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTGATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAGCGgcgcgcg                                                                     <  2:483481/133‑2 (MQ=255)                                       gccgccACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTa                                                                   <  1:389545/130‑1 (MQ=255)                                       gccgccACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTa                                                                   >  2:389545/1‑130 (MQ=255)                                          gccACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCcaca                                                             <  2:382550/133‑1 (MQ=255)                                            cACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAg                                                           >  1:263507/1‑133 (MQ=255)                                              ccGGGCCATGTGTGTGAGTTATGCGGATTGCGGCAACACTCCCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAggg                                                         <  2:172993/133‑1 (MQ=255)                                                  gCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTtgt                                                     >  2:333544/1‑133 (MQ=255)                                                     aTGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTa                                                  <  1:333544/133‑1 (MQ=255)                                                      tGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTaa                                                 >  1:261726/1‑133 (MQ=255)                                                                     cAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACt                                  >  1:459998/1‑133 (MQ=255)                                                                      aGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTa                                 <  2:459998/133‑1 (MQ=255)                                                                        aTTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTaaa                               >  1:111193/1‑133 (MQ=255)                                                                            cGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCCACCCACAAGGGTTGTGGAAATCCCAATGGGGACTAAACGaa                           >  2:191887/1‑133 (MQ=255)                                                                             ggCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTCCCCACAAGGGGTATGTAAATCCCACGGGGGGCTAAACGAAg                          >  2:162853/1‑133 (MQ=255)                                                                              gCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGt                         <  2:55121/133‑1 (MQ=255)                                                                                      cgcgCCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTtgtg                 <  2:111193/133‑1 (MQ=255)                                                                                        cgcCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTtgtgtg               <  1:463400/133‑1 (MQ=255)                                                                                        cgcCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTtgtgtg               <  1:416905/133‑1 (MQ=255)                                                                                        cgcCTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTtgtgtg               <  2:61858/133‑1 (MQ=255)                                                                                          ccTTTACCTGCCCGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGga             <  2:126545/133‑1 (MQ=255)                                                                                                   gCCCGTATAACGGCTCGCTTTATGGCATTATCGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATaa    <  1:162853/133‑1 (MQ=255)                                                                                                     ccGTATCACGGCTGGCCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGGCTACCCACAAGGGGTGTGTAAATCCCGCTGTGGACGAAAAGAAATTCCTTGTATGGACAGTTATAAAg  >  2:159612/1‑133 (MQ=255)                                                                                                                    |                                                                                                                      TCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAG  >  NC_000913/2669247‑2669479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑