Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,710,853	T→C	V145A (GTG→GCG)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,710,853	0	T	C	100.0%	44.7 / NA	14	V145A (GTG→GCG)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/5);  total (9/5)

AATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATGGAACCG  >  NC_000913/2710750‑2710979                                                                                                        |                                                                                                                               aaTGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATAttt                                                                                                   >  2:8467/1‑132 (MQ=255)                         aCCCGTGAAGATGGACATAGTCACCGTCTTATTCTTCATGCGGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCgg                                                                           <  2:154971/133‑1 (MQ=255)                          cccGTGAAGGTGGACCTAGTCACCTTCGCATTCTTCATGCCACCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGt                                                                          <  2:146063/133‑1 (MQ=255)                          cccGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGt                                                                          <  2:43193/133‑1 (MQ=255)                                aaGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCt                                                                    >  2:290537/1‑133 (MQ=255)                                      gACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGCGCGCAGCAACGCGGTTGATCTGATTGt                                                              >  2:350279/1‑133 (MQ=255)                                          tAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCATCGCGGTGGATCTGTTTGTTTCt                                                          >  1:145052/1‑133 (MQ=255)                                                 cgtcgtATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAAAGCGATTGATCTGGTTGTATCTGACaaaa                                                   >  2:146883/1‑133 (MQ=255)                                                 cgtcgtATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCAGGAGCGCAGCAATGCGTTTGATCTGGTTGTGTCTGACaaaa                                                   >  2:186902/1‑133 (MQ=255)                                                 cgtcgtATTCTTCATGCCGCCGACGCCACCGGCAGAGAGGTAGAAGCCACCCTGGCGAGCAAGGCGCTGAACCACCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTGGATCTGATTGCTTCTGACaaaa                                                   >  2:22809/1‑133 (MQ=255)                                                                gccgccGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCa                                    >  2:92524/1‑133 (MQ=255)                                                                                    agaAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGcgcg                <  1:483031/133‑1 (MQ=255)                                                                                     gaAGTAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTTGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGt               <  1:8467/133‑1 (MQ=255)                                                                                         tAGAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGGGACTGGATGTTGGAGCGTGGGt           >  1:244296/1‑133 (MQ=255)                                                                                           gAAACCACGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTAt         >  2:5369/1‑133 (MQ=255)                                                                                                  cGCTGGCGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATGGAACCg  >  2:39761/1‑133 (MQ=255)                                                                                                        |                                                                                                                               AATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATGGAACCG  >  NC_000913/2710750‑2710979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑