Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,976	A→G	H417R (CAC→CGC)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,976	0	A	G	100.0%	59.5 / NA	18	H417R (CAC→CGC)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

ACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTC  >  NC_000913/2723853‑2724095                                                                                                                            |                                                                                                                        aCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGa                                                                                                                <  2:16457/133‑1 (MQ=255)                        ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATc                                                                                         >  1:78128/1‑133 (MQ=255)                                cGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAg                                                                                 >  2:494105/1‑133 (MQ=255)                                cGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAg                                                                                 <  2:21410/133‑1 (MQ=255)                                    aTTTTGATCCGCGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTc                                                                             <  2:201940/133‑1 (MQ=255)                                     ttttGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCCAAGTATTGCCGTTTCTCCGGTGAAGGTTCg                                                                            >  1:158261/1‑133 (MQ=255)                                               cGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTACCGGTTAGCCGGTGAACGTTCATAAACTCATc                                                                  >  2:188885/1‑133 (MQ=255)                                                gATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATcc                                                                 >  1:308357/1‑133 (MQ=255)                                                         cTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgc                                                        >  1:501285/1‑133 (MQ=255)                                                            gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGcc                                                     >  2:401403/1‑133 (MQ=255)                                                               cTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTa                                                  <  1:401403/133‑1 (MQ=255)                                                                         aGCGAGAGTAAGAACTGGGGCTTATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCACCGTTTGCGCCGTATCCGCATCGa                                        <  1:171487/133‑1 (MQ=255)                                                                            gagagaAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCg                                     <  2:501285/133‑1 (MQ=255)                                                                             agagaAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGa                                    <  1:32867/133‑1 (MQ=255)                                                                                              tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCt                   <  2:78128/133‑1 (MQ=255)                                                                                                     cgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATAGACCGATTAATAAGTCGCATCCTGTAATca            >  1:188791/1‑133 (MQ=255)                                                                                                            accaccATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcccc     >  1:373469/1‑133 (MQ=255)                                                                                                               accaTCGTTAGGCGCTATCGCGATATGCTAAGAATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc  <  2:188791/133‑1 (MQ=255)                                                                                                                            |                                                                                                                        ACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTC  >  NC_000913/2723853‑2724095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑