Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,523	T→C	V34V (GTA→GTG)	yadS ←	conserved inner membrane protein YadS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,523	0	T	C	100.0%	77.4 / NA	23	V34V (GTA→GTG)	yadS	conserved inner membrane protein YadS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

CGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGTACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTG  >  NC_000913/177396‑177648                                                                                                                                |                                                                                                                              caaTGGTCAGAATGCGGGCGACCGTTACTACGACCAGAACGCTGGGATCTTTCACCCAAAATCCCGAGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTTCCACGCCCAGCACCAg                                                                                                                          <  2:243575/131‑1 (MQ=255) cGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAg                                                                                                                          >  1:302363/1‑133 (MQ=255)               cTGGTGACCATTGCACCGCCCACATCGGTGCGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGt                                                                                                            <  1:251782/133‑1 (MQ=255)               cTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGt                                                                                                            <  2:302363/133‑1 (MQ=255)                tGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTc                                                                                                           >  1:66514/1‑133 (MQ=255)                     aCCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATAc                                                                                                      >  2:415511/1‑133 (MQ=255)                     aCCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATAc                                                                                                      >  1:326870/1‑133 (MQ=255)                                        cGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCACGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACaa                                                                                   <  1:424091/133‑1 (MQ=255)                                          gTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACaaaa                                                                                 >  2:272146/1‑133 (MQ=255)                                                   ttCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGgaga                                                                        >  2:463663/1‑133 (MQ=255)                                                     cACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGAAATTTTCCGGCAAACAAAACGCCGGAGATg                                                                      >  1:250501/1‑133 (MQ=255)                                                                    gCCGTGATCCAGCGCTATGTCGCGCATTTTCCGGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCtgtg                                                       <  2:220317/133‑1 (MQ=255)                                                                    gCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCtgtg                                                       >  1:5085/1‑133 (MQ=255)                                                                     ccGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGc                                                      <  1:415511/133‑1 (MQ=255)                                                                             cAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTGACAAAACGTCGGTGATGGCAAATACGGCTGAGCCGACtata                                              >  2:134778/1‑133 (MQ=255)                                                                               gcgcCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATc                                            >  2:471323/1‑133 (MQ=255)                                                                                     tGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCa                                      >  2:424723/1‑133 (MQ=255)                                                                                      gTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCaa                                     <  1:39549/133‑1 (MQ=255)                                                                                      gTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCaa                                     <  1:330046/133‑1 (MQ=255)                                                                                      gTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCaa                                     <  2:66514/133‑1 (MQ=255)                                                                                         gcgAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAg                                  <  2:326870/133‑1 (MQ=255)                                                                                             aTTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGa                              >  2:294656/1‑133 (MQ=255)                                                                                                                 ggTTACCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTg          <  1:272146/133‑1 (MQ=255)                                                                                                                     aCCACGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCa      >  1:119396/1‑133 (MQ=255)                                                                                                                         cGCCCAGCACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAACACCTg  <  1:471323/133‑1 (MQ=255)                                                                                                                                |                                                                                                                              CGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGCGCCATGTCGCGAATTGTCCCGCCGCCTACTGCGGTTACCACGCCCAGTACCAGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTG  >  NC_000913/177396‑177648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑