Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,741,920	A→G	T16A (ACG→GCG)	yfiR →	DUF4154 domain‑containing protein YfiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,741,920	0	A	G	100.0%	58.3 / NA	19	T16A (ACG→GCG)	yfiR	DUF4154 domain‑containing protein YfiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

CGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTT  >  NC_000913/2741792‑2742045                                                                                                                                 |                                                                                                                              cGGTCGCACGAAAAAGTGCTATATTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCggg                                                                                                                           <  1:243777/133‑1 (MQ=255)   gTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTTTCACCGACTGTTATAACGTCTGATTTTCCTTATGGCgggcg                                                                                                                         >  2:243777/1‑131 (MQ=255)   gTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGATCTTACTGCTGATTCTCCTGCTGGCGGGAg                                                                                                                         >  1:206001/1‑133 (MQ=255)       cATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCAcc                                                                                                                     >  2:262810/1‑133 (MQ=255)       cATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCAc                                                                                                                      >  2:444715/1‑132 (MQ=255)       cATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCAc                                                                                                                      <  1:444715/132‑1 (MQ=255)                                cATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGAc                                                                                            >  2:290440/1‑133 (MQ=255)                                              atCGATGCGTTTCGACCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGt                                                                              <  1:205211/133‑1 (MQ=255)                                                      aTTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTatga                                                                      <  2:87764/133‑1 (MQ=255)                                                       tttCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTatgat                                                                     >  2:106232/1‑133 (MQ=255)                                                          cGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGt                                                                  <  2:454426/133‑1 (MQ=255)                                                                                  gAATGCGTTTTTCTCACCGACTGTACTTTCTTCTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCg                                          <  2:215261/133‑1 (MQ=255)                                                                                      gCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGGCGGGAGCACCAATCCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTgg                                      <  2:77661/133‑1 (MQ=255)                                                                                                cACCGACTGTGCTTATTTCTGATTTTCCTGCTCGCGGGAGCTCCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTAt                            <  2:151811/133‑1 (MQ=255)                                                                                                           cTTACTTCTGGTTCTTCTGCTGGCGGGACCACTAATACTCGTTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGccacca                 <  2:206001/133‑1 (MQ=255)                                                                                                                  cTGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTAt          <  1:298582/133‑1 (MQ=255)                                                                                                                   tGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATg         >  1:85694/1‑133 (MQ=255)                                                                                                                   tGATTCTCCTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATg         >  1:383521/1‑133 (MQ=255)                                                                                                                          ccTGCTGGCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTAGGTATCttt  >  1:228968/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                              CGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTT  >  NC_000913/2741792‑2742045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑