Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,745,169 T→C G57G (GGA→GGG)  trmD ← tRNA m(1)G37 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,745,1690TC100.0% 112.6 / NA 34G57G (GGA→GGG) trmDtRNA m(1)G37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

CTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCG  >  NC_000913/2745041‑2745298
                                                                                                                                |                                                                                                                                 
ttgccGCCCGCTTGATCAAGCTGGCGTCCCTGTGGTGAAAGATAAATCCCCTTGGCCCTTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCccgc                                                                                                                               <  2:413456/129‑1 (MQ=255)
ttGACGCCCGTTTGACCAGGTTTGGGTCCGTGTGGGGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCccgc                                                                                                                               <  2:481389/132‑1 (MQ=255)
cTGACGCCCGCTTGAACACGCTCGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCccgc                                                                                                                               <  1:195171/133‑1 (MQ=255)
      cccGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGt                                                                                                                         >  1:491195/1‑133 (MQ=255)
      cccGCTTGATCAACCTGGCTCCCTTGGGGTGAAAAATAAACCCCTTTTCCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGt                                                                                                                         <  2:20526/133‑1 (MQ=255)
                        cGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCacgg                                                                                                       >  1:392048/1‑133 (MQ=255)
                                  gtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCg                                                                                             <  2:39100/133‑1 (MQ=255)
                                     aCAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTc                                                                                          >  2:273831/1‑133 (MQ=255)
                                        gATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATg                                                                                       >  2:269141/1‑133 (MQ=255)
                                                    ttGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCGACGATACGGTGCCGGTGATGCTTGAAGTcgcg                                                                           >  2:162446/1‑133 (MQ=255)
                                                    ttGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                           >  1:358814/1‑133 (MQ=255)
                                                      gcgcCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAg                                                                         <  2:392048/133‑1 (MQ=255)
                                                      ccgcCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAg                                                                         <  2:80284/132‑1 (MQ=255)
                                                       cgcCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAg                                                                         <  1:407402/132‑1 (MQ=255)
                                                       cgcCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAg                                                                         >  2:407402/1‑132 (MQ=255)
                                                            tCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTcc                                                                   >  2:304548/1‑133 (MQ=255)
                                                            tCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTcc                                                                   >  1:281311/1‑133 (MQ=255)
                                                                 cgccgcGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGctc                                                              <  1:262349/133‑1 (MQ=255)
                                                                 cgccgcGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGCCATGCGTGAAGTCGCGAGGACTCCAGctc                                                              >  1:328443/1‑133 (MQ=255)
                                                                       ggCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATg                                                        <  1:273831/133‑1 (MQ=255)
                                                                                tgcATGAATGGCGTCGCGTAAGCGTTGCACTATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAgg                                               <  1:162446/133‑1 (MQ=255)
                                                                                         ggCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTa                                      <  1:416828/133‑1 (MQ=255)
                                                                                         ggCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTa                                      <  1:304548/133‑1 (MQ=255)
                                                                                                cGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGccc                               >  2:18172/1‑133 (MQ=255)
                                                                                                cGCAAGGGTTGCACCATCATTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCAGGATGCTAAGCAGGCCATTTTGAACTGccc                               >  1:249833/1‑133 (MQ=255)
                                                                                                       gTTGCACCATCATTACCATCCCCGCCCCCCCGCCGTAAGGAAGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGt                        <  2:202235/133‑1 (MQ=255)
                                                                                                              catcatTACGCTCCCAGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCg                 <  2:249833/133‑1 (MQ=255)
                                                                                                              catcatTACCATCCCCGGCCCGCCGCCGTATGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCg                 <  1:145942/133‑1 (MQ=255)
                                                                                                                 catTAACATCCCCGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTaa              >  1:40487/1‑133 (MQ=255)
                                                                                                                          ccccGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTACCTTACTGCACAGTTACACCGTCATCGGTacat     >  2:240405/1‑130 (MQ=255)
                                                                                                                          ccccGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAAtt     >  1:351545/1‑133 (MQ=255)
                                                                                                                          ccccGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAAtt     >  1:377415/1‑133 (MQ=255)
                                                                                                                           cccGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTg    >  1:122496/1‑133 (MQ=255)
                                                                                                                             cGGCCCGCCGCCGTACGGACGACCGTCCACCGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg  <  1:148397/133‑1 (MQ=255)
                                                                                                                             cGGCCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg  <  1:269141/133‑1 (MQ=255)
                                                                                                                                |                                                                                                                                 
CTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCG  >  NC_000913/2745041‑2745298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑