Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,758,296 T→C H102R (CAC→CGC)  yfjH ← CP4‑57 prophage; protein YfjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,758,2960TC100.0% 111.2 / NA 32H102R (CAC→CGC) yfjHCP4‑57 prophage; protein YfjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CTCGATAACAAGAGGTAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACAT  >  NC_000913/2758169‑2758425
                                                                                                                               |                                                                                                                                 
cTCGATAACAAGAGGTAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCtt                                                                                                                              >  1:414744/1‑133 (MQ=255)
 tCGATAACAGCAGGTGAAAACGTTTCCTTGGTCAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTg                                                                                                                             <  2:247278/133‑1 (MQ=255)
   gATAACAAGAGGTAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGtt                                                                                                                           <  2:276260/133‑1 (MQ=255)
        cAAGAGGTAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCa                                                                                                                      <  2:414744/133‑1 (MQ=255)
               tAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGAATTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACa                                                                                                               >  2:435689/1‑133 (MQ=255)
                aaaaaCGTGTCCTTGGTATGGTTTGAACTTTGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGTCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACAt                                                                                                              <  1:210380/133‑1 (MQ=255)
                   aaCGTTTCCTTGGTATGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGc                                                                                                           <  1:173441/133‑1 (MQ=255)
                       tttCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGc                                                                                                           >  2:256486/1‑129 (MQ=255)
                       tttCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGc                                                                                                           <  1:256486/129‑1 (MQ=255)
                                    gTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTcaca                                                                                          <  2:421204/133‑1 (MQ=255)
                                         aaCTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGt                                                                                     >  2:436157/1‑133 (MQ=255)
                                         aaCTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGt                                                                                     >  1:1589/1‑133 (MQ=255)
                                         aaCTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTGGTTTCCCAGGGTTCATAGCAGGAATGTAACATCACACGAGt                                                                                     >  2:159441/1‑133 (MQ=255)
                                          aCTTAGAATTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTa                                                                                    <  1:435689/133‑1 (MQ=255)
                                                       cAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCag                                                                       <  2:1589/133‑1 (MQ=255)
                                                        aGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACGTCGCAGGAGTATGACCTCACACGAGTACCTGTTTTCTCaga                                                                      >  2:196779/1‑133 (MQ=255)
                                                          cATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGc                                                                    >  2:290966/1‑133 (MQ=255)
                                                                                 ttCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAAAGCTCCATAATTGCGCTCGCATTCTg                                             >  2:474889/1‑133 (MQ=255)
                                                                                      tgtATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACTTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCAtt                                                <  2:331455/125‑1 (MQ=255)
                                                                                      tgtATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCAtt                                                >  1:331455/1‑125 (MQ=255)
                                                                                      tgtATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAg                                        >  2:19974/1‑133 (MQ=255)
                                                                                      tgtATTCTGCCTTTAGCTCCAGTTGCCCATCCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAg                                        <  1:196779/133‑1 (MQ=255)
                                                                                          ttcttcCTGTATCTCCAGCTGCCCATTCACCGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCa                                    <  1:159441/133‑1 (MQ=255)
                                                                                                cTTTATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGt                              >  1:20591/1‑133 (MQ=255)
                                                                                                  ttATCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTAt                            >  1:92010/1‑133 (MQ=255)
                                                                                                    aTCTCCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTAttt                          >  2:61101/1‑133 (MQ=255)
                                                                                                       tcCAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCg                       <  1:290966/133‑1 (MQ=255)
                                                                                                         cAGTTGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGga                     <  1:19974/133‑1 (MQ=255)
                                                                                                             tGCCCATTCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGgatgat                 >  1:37794/1‑133 (MQ=255)
                                                                                                                   ttCACTGTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTCGCTCAATGCGTGTTCCCGGATAATCTTCct           >  1:184654/1‑133 (MQ=255)
                                                                                                                         gTATGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa     <  1:325647/133‑1 (MQ=255)
                                                                                                                           aTGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACa   <  2:20591/133‑1 (MQ=255)
                                                                                                                            tGGCGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACAt  >  1:299825/1‑133 (MQ=255)
                                                                                                                               |                                                                                                                                 
CTCGATAACAAGAGGTAAAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACAT  >  NC_000913/2758169‑2758425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑