| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,804,405 | T→C | S302P (TCC→CCC) | nrdF → | ribonucleoside‑diphosphate reductase 2 subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,804,405 | 0 | T | C | 94.4% | 55.9 / NA | 18 | S302P (TCC→CCC) | nrdF | ribonucleoside‑diphosphate reductase 2 subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (8/9); minor base A (1/0); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAA > NC_000913/2804276‑2804534 | aaTAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGcccct > 2:455819/1‑133 (MQ=255)aaTAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGcccct > 2:402760/1‑133 (MQ=255) gCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCCCGAATGCATTTCCGGTTCAGGCACCTCTTAtg > 2:232892/1‑133 (MQ=255) tttGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTAtgtg > 1:32247/1‑133 (MQ=255) ttGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGa > 2:376593/1‑133 (MQ=255) tggtgaATCTGGGCTACAAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGAt > 2:448112/4‑133 (MQ=255) tGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGc < 1:455819/133‑1 (MQ=255) gCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGt > 2:400942/1‑133 (MQ=255) aaCCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAAc < 1:402760/133‑1 (MQ=255) aCCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACa < 1:376593/133‑1 (MQ=255) aTTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGAt < 2:32247/133‑1 (MQ=255) cccGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAg > 1:393759/1‑133 (MQ=255) cAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTg < 1:400942/133‑1 (MQ=255) aGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAggg > 1:387500/1‑133 (MQ=255) ccgcGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCa > 1:469870/1‑133 (MQ=255) gCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCgcag < 2:387500/133‑1 (MQ=255) ccGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCgcagc > 2:90130/1‑133 (MQ=255) gAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGcagcag < 1:448112/133‑1 (MQ=255) aGGCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCa < 2:477678/133‑1 (MQ=255) ggCCCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCaa < 1:90130/133‑1 (MQ=255) | AATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAA > NC_000913/2804276‑2804534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |