Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,809,853	T→C	A79A (GCT→GCC)	ygaZ →	L‑valine exporter subunit YgaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,853	0	T	C	100.0%	87.0 / NA	26	A79A (GCT→GCC)	ygaZ	L‑valine exporter subunit YgaZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/18);  total (8/18)

TCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCGCCCTGT  >  NC_000913/2809727‑2809953                                                                                                                               |                                                                                                     tCTGAATGCGTCCCGACTGGGATTCTCTGCTCCCGAAAGCGGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTg                                                                                                <  2:145792/133‑1 (MQ=255)      aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGt                                                                                           <  2:274749/133‑1 (MQ=255)       tGCGCCCCGTCTGGGTTTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTGCCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTc                                                                                          <  1:509103/133‑1 (MQ=255)       tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTc                                                                                          <  1:366276/133‑1 (MQ=255)           aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATgg                                                                                      >  2:290137/1‑133 (MQ=255)                     gATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGt                                                                            <  1:486869/133‑1 (MQ=255)                     gATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGt                                                                            <  2:1132/133‑1 (MQ=255)                      atacgctcctctcGAAAGCGTTTTTTCCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGtt                                                                           <  2:293811/128‑1 (MQ=255)                          tctcctctcGCAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGCGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAGTGGATGTTCGcc                                                                       >  2:272074/1‑133 (MQ=255)                            tcctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGGTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCAt                                                                     <  2:456802/133‑1 (MQ=255)                             cctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCAtg                                                                    <  2:288721/133‑1 (MQ=255)                              ctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGcc                                                                       <  2:31949/129‑1 (MQ=255)                              ctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGcc                                                                       >  1:31949/1‑129 (MQ=255)                                         gTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGcc                                                        <  2:481922/133‑1 (MQ=255)                                               ttCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCAc                                                  <  2:344152/133‑1 (MQ=255)                                                    cTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGt                                             >  1:361920/1‑133 (MQ=255)                                                    cTGCATCATTTATGCAGGAGCGGGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGGCCGTCACTGCGt                                             <  1:205840/133‑1 (MQ=255)                                                      gCATCATTTATGCAGTCGCGAGCCAGTTTGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAg                                           <  2:194957/133‑1 (MQ=255)                                                            tttATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGtat                                     >  1:18424/1‑133 (MQ=255)                                                                 gCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTc                                >  2:268014/1‑133 (MQ=255)                                                                    ggCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGc                             >  2:463713/1‑133 (MQ=255)                                                                                     cATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGGCACTGCGTAGCCTTATCATTCAGCGGCTGCAAAAATCGaaa            >  2:188510/1‑133 (MQ=255)                                                                                         aCCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCg        <  1:411849/133‑1 (MQ=255)                                                                                         aCCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCg        <  1:290137/133‑1 (MQ=255)                                                                                           cgcgATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCACACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCGcc      <  2:13120/133‑1 (MQ=255)                                                                                           cgcgATGCTGGCAGCCGGGAGTAGTTTGTGGATCGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCGcc      <  1:268014/133‑1 (MQ=255)                                                                                               aTGCTGGCAGCCGGGAGTAGTTTGTGGATTGCCGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTGAGCGTCTGCAAGAATCGAAAAGCGCCCtgt  >  1:176322/1‑133 (MQ=255)                                                                                                                               |                                                                                                     TCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACTGCGTAGCCGTATTATTCAGCGTCTGCAAAAATCGAAAACCGCCCTGT  >  NC_000913/2809727‑2809953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑