| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,829,702 | T→C | V219A (GTT→GCT) | srlR → | DNA‑binding transcriptional repressor SrlR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,829,702 | 0 | T | C | 96.4% | 86.4 / ‑4.0 | 28 | V219A (GTT→GCT) | srlR | DNA‑binding transcriptional repressor SrlR |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (13/14); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
TCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGTTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATAACCGGAGAGAGCAATGAG > NC_000913/2829573‑2829817 | tCGATCTCAATGCGGACGTAACCAACTTTAACAAGGTTTATCCCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTg < 2:175919/133‑1 (MQ=255) ctcAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTc > 1:263799/1‑133 (MQ=255) cGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAg < 1:458329/133‑1 (MQ=255) ggCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCg > 2:22659/1‑133 (MQ=255) ggCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCg > 1:358530/1‑133 (MQ=255) ggCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCg > 1:400650/1‑133 (MQ=255) accaccTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATa < 1:22659/133‑1 (MQ=255) cGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTAcc < 1:52403/133‑1 (MQ=255) tCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGACTTGAAAGCGTCAATATGCTGATTACCGACCCAGGTATCGa > 2:187142/1‑133 (MQ=255) tgtgCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTGGATAAGCTTATTGCCGGCGCAGGTAGCGATACGGCGTTTCg > 2:138521/1‑133 (MQ=255) tgCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGGTAAGCTGAGTACCGACGCAGGTATCAATCCGGCGTTTCGTc > 2:155333/1‑133 (MQ=255) tgCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCTGGTTTCTATCCGGTGTTTCGTc > 1:201675/1‑133 (MQ=255) aaTGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAgg < 2:451938/133‑1 (MQ=255) ccGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCt < 2:400650/133‑1 (MQ=255) cgcgcgAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGGAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGa > 2:334019/1‑133 (MQ=255) cgcgcgAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGTTATCGATTCGGGGTGTCGTCAAGCGCTGGa > 1:194552/1‑133 (MQ=255) gcgcgAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTg > 1:386240/1‑130 (MQ=255) gcgcgAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTg < 2:386240/130‑1 (MQ=255) cgcgAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAag > 2:476016/1‑133 (MQ=255) aaGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGaa < 1:476016/133‑1 (MQ=255) ttttGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGAt < 2:353167/133‑1 (MQ=255) ttttGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGAt < 1:122471/133‑1 (MQ=255) gATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGAt < 1:334019/133‑1 (MQ=255) gATGGCGGACTCATCAAAATTTGGCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGAt < 1:155333/133‑1 (MQ=255) tcatcaAAGTTTGGCTGTAAAAGCCCCACCGTTGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATaa < 2:201675/133‑1 (MQ=255) ggCCGTAAAAGCCCCAACGTAGTTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATAACCGGAGAGAGCa > 2:278322/1‑133 (MQ=255) ggCCGTAAAAGCCCCAACGTAGCTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATAACCGGAGAGAGCa > 2:82167/1‑133 (MQ=255) tAAAAGCCACAACGTCGCATGCCGTCTTGACAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATAATAACCGGAGAGAGCAATGAg < 1:138521/133‑1 (MQ=255) | TCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGTTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATAACCGGAGAGAGCAATGAG > NC_000913/2829573‑2829817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |