Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	203,098	A→G	D180G (GAC→GGC)	lpxA →	UDP‑N‑acetylglucosamine acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	203,098	0	A	G	100.0%	70.0 / NA	22	D180G (GAC→GGC)	lpxA	UDP‑N‑acetylglucosamine acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

AACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGA  >  NC_000913/202971‑203223                                                                                                                                |                                                                                                                              aacgcaacgcTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTcc                                                                                                                          <  2:424356/133‑1 (MQ=255) aacgcaacgcTGGCGAGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTcc                                                                                                                          <  1:214971/133‑1 (MQ=255)                                       cgcgATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCg                                                                                    >  2:373554/1‑133 (MQ=255)                                                      cATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATc                                                                     >  2:425800/1‑133 (MQ=255)                                                        tGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGa                                                                   >  1:229758/1‑133 (MQ=255)                                                                      cAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCGCGCAACGCCGTTCGGTGGCAATATCGAAGGGCTGAAGcgcc                                                     <  2:339550/133‑1 (MQ=255)                                                                              catcatTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAATCACGCAACGCCGTTCGGTATCAATATCGAAGGGCTGAAGCGCCGCGGATTc                                             >  2:419673/1‑133 (MQ=255)                                                                                    tGGTGCGCCCGTGATAGTCGGCTGCTGCTCTGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGt                                       <  1:252913/133‑1 (MQ=255)                                                                                         cgcACGTGATAGTTGTCGGCTGCTTCGGGGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCATCGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGc                                  <  2:188695/133‑1 (MQ=255)                                                                                           cACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGa                                >  1:280307/1‑133 (MQ=255)                                                                                           cACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGAGCTTAAGGGCCGCGGATTCAGGCGTGAGGCGa                                >  2:170439/1‑133 (MQ=255)                                                                                                  tGGTTGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGc                         <  1:373554/133‑1 (MQ=255)                                                                                                      tGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATc                     >  2:348957/1‑133 (MQ=255)                                                                                                      tGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATc                     >  1:345156/1‑133 (MQ=255)                                                                                                      tGGCGGCTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGAGGATTCAGCCGAGACGCGATTACCGCTATc                     >  1:156268/1‑133 (MQ=255)                                                                                                        gcggcTGCTCCGGTGTGGCGCAGGGCGTCCC‑CCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGc                  >  2:419236/1‑133 (MQ=255)                                                                                                          ggcTGCTCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCa                 <  1:491887/133‑1 (MQ=255)                                                                                                            ctgctCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCa                 <  1:429507/131‑1 (MQ=255)                                                                                                            ctgctCCGGTGTGGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCa                 >  2:429507/1‑131 (MQ=255)                                                                                                                      gtgGCGCAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGc     <  2:345156/133‑1 (MQ=255)                                                                                                                       tgGCGCAGGGCGTCCC‑CCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTg   <  1:419236/133‑1 (MQ=255)                                                                                                                         gcgcAGGGCGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCACCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGa  >  2:200189/1‑133 (MQ=255)                                                                                                                                |                                                                                                                              AACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGA  >  NC_000913/202971‑203223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑