Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,697	A→G	G121G (GGA→GGG)	ygbK →	putative 3‑oxo‑tetronate kinase YgbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,697	0	A	G	100.0%	85.5 / NA	26	G121G (GGA→GGG)	ygbK	putative 3‑oxo‑tetronate kinase YgbK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/8);  total (18/8)

TATTTCAAATACTGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGC  >  NC_000913/2862572‑2862815                                                                                                                              |                                                                                                                          tATTTCAAATACTGCTCTACTTTCGACAGTACGGCGAAAGGTAATA‑TGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGt                                                                                                                   <  2:83874/133‑1 (MQ=255)        aaTACTGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGGCCTGCCGATCAGCGAGCGTACTGTTTATCa                                                                                                             >  1:134770/1‑133 (MQ=255)        aaTACTGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCa                                                                                                             >  2:286084/1‑133 (MQ=255)             tGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGt                                                                                                        >  2:408910/1‑133 (MQ=255)             tGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGt                                                                                                        >  1:453210/1‑133 (MQ=255)              gCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCAGGTCAACGGGCGTAGGGTTTACCAGGGGTa                                                                                                       >  1:138049/1‑133 (MQ=255)               ctctACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAt                                                                                                      >  2:83813/1‑133 (MQ=255)               ctctACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAt                                                                                                      >  2:304342/1‑133 (MQ=255)                       tCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGt                                                                                           <  1:329110/133‑1 (MQ=255)                              tACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAAt                                                                                    <  1:462672/133‑1 (MQ=255)                                 ggCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCaa                                                                                 >  1:90977/1‑133 (MQ=255)                                 ggCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCaa                                                                                 >  2:343519/1‑133 (MQ=255)                                                  gCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCgg                                                                >  1:471113/1‑133 (MQ=255)                                                  gCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTGCGTAATGTATCAACAGCTGGCAGAATCCgg                                                                >  1:230986/1‑133 (MQ=255)                                                   cccGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCggg                                                               >  1:56170/1‑133 (MQ=255)                                                       gTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATgc                                                           >  1:372523/1‑133 (MQ=255)                                                               tGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCAc                                                   <  1:408910/133‑1 (MQ=255)                                                               tGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCAc                                                   <  1:343519/133‑1 (MQ=255)                                                                 ccTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTATTTGTTGTTCGTAATGAATCAACTGCTGGCCGTATACGGGATACGCCATCa                                                    >  1:244588/1‑133 (MQ=255)                                                                     aaTGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGCATGCGCCATAACCCGGTa                                             >  1:187114/1‑133 (MQ=255)                                                                           tGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATccc                                       >  1:444951/1‑133 (MQ=255)                                                                            gCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCa                                      >  2:357663/1‑133 (MQ=255)                                                                                         cGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTa                         <  2:453210/133‑1 (MQ=255)                                                                                                  tcttctCTCCGGCCCTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCg                <  1:468052/133‑1 (MQ=255)                                                                                                              cccTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATTACCGACAACTATCTTCCCCGTCCGGTTGAAgc    >  2:233726/1‑133 (MQ=255)                                                                                                                cTGCCGGTCAACGGGCGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAgcgc  <  2:372523/133‑1 (MQ=255)                                                                                                                              |                                                                                                                          TATTTCAAATACTGCTCTACTTTCGACAGTACGGCGAAAGGTAATATTGGCCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTAT‑‑‑TTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGC  >  NC_000913/2862572‑2862815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑