| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,867,559 | C→T | intergenic (‑8/+55) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/murein hydrolase activator NlpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,867,559 | 0 | C | T | 100.0% | 63.4 / NA | 20 | intergenic (‑8/+55) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/murein hydrolase activator NlpD |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/9); total (11/9) | |||||||||||
GCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGT > NC_000913/2867430‑2867683 | gCCAAATCGTTATCACTGAGTTCCTGTTCTTCTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTcc < 1:216879/133‑1 (MQ=255) cTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAAc > 2:89869/1‑133 (MQ=255) ttCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCCTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACAtt > 1:331787/1‑133 (MQ=255) actaAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCAAAAGGTGGTGCCTACCCGTGATCCCTAGACGAAACATTCAAg > 2:139994/1‑133 (MQ=255) aaGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCaa < 2:512095/133‑1 (MQ=255) aaGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCaa < 2:285528/133‑1 (MQ=255) gCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAg > 1:312456/1‑127 (MQ=255) gCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAg < 2:312456/127‑1 (MQ=255) gCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAg > 1:36622/1‑133 (MQ=255) ttttCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGGTCCCTTGGCGGAACTTTCAAGGAAAAGCCt > 1:136294/1‑133 (MQ=255) tCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGt < 1:89869/133‑1 (MQ=255) atcaAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt > 2:377449/1‑133 (MQ=255) atcaAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt < 2:36622/133‑1 (MQ=255) ccGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGc < 1:449740/133‑1 (MQ=255) gACTCATACGGTGGTACCCACCAGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGc < 1:139994/133‑1 (MQ=255) cATAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGc > 1:68690/1‑133 (MQ=255) tAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTTCCCTTGTTACGAATTTCAAAATGCAAGCgt > 1:252875/1‑133 (MQ=255) tAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAg < 2:376528/130‑1 (MQ=255) tAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAg > 1:376528/1‑130 (MQ=255) tAAGGTGGTTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCgt > 1:15589/1‑133 (MQ=255) | GCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGT > NC_000913/2867430‑2867683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |