Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,893,121	T→C	G155G (GGT→GGC)	ygcN →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,893,121	0	T	C	100.0%	70.7 / NA	22	G155G (GGT→GGC)	ygcN	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

CATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAAT  >  NC_000913/2893013‑2893250                                                                                                             |                                                                                                                                  cATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGt                                                                                                           <  1:293988/133‑1 (MQ=255)    ccccGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCAc                                                                                                        >  1:113331/1‑133 (MQ=255)    ccccGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCAc                                                                                                        <  1:6336/133‑1 (MQ=255)             gACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTg                                                                                               <  1:348746/133‑1 (MQ=255)                         aCTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCctgct                                                                                   >  1:442260/1‑133 (MQ=255)                             tATGAAGACAACGGCCGAGTCTGTGGCGTTGTTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCg                                                                               <  1:214987/133‑1 (MQ=255)                               tGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTTACTCGTCCTCCTGGCGaa                                                                             >  1:177920/1‑133 (MQ=255)                                       aCGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGc                                                                     >  1:392773/1‑133 (MQ=255)                                        cGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCg                                                                    <  2:331960/133‑1 (MQ=255)                                          gCAGTGTCCGTGGCGCTACTTGTGTTTATGATCTTCTCCGGCCCCGTTATGTGGTGGTGGCGGAAGGCGCAAACAGCGTCGTGGCTGAACGTCACCGGTTAGTGACTCGGCCTGGTGGCGAAGCGATGGCGtt                                                                  <  2:240427/133‑1 (MQ=255)                                          gCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGtt                                                                  <  2:442260/133‑1 (MQ=255)                                           cAGAGTCTATGGCGTTATCTGTAGTGACGAGATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTAACTCGTCCTGCTGGCGAAGCGATGGCGTTg                                                                 <  1:218813/133‑1 (MQ=255)                                           cAGAGTCTATGGCGTGATTTGTCGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTg                                                                 <  1:236406/133‑1 (MQ=255)                                                   gtgGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCaa                                                         >  2:290060/1‑133 (MQ=255)                                                          tATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTg                                                  >  2:189115/1‑133 (MQ=255)                                                                   tgACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTg                                         >  1:320074/1‑133 (MQ=255)                                                                           ttCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATc                                 <  2:113331/133‑1 (MQ=255)                                                                             ctcCGTGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTTACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACTTCCg                               <  2:395793/133‑1 (MQ=255)                                                                             ctcCGCGCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCg                               <  1:290060/133‑1 (MQ=255)                                                                                cgcgCCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCGAAGAAGTCCTGACGATGGAAACATCCGCTa                            >  1:245753/1‑133 (MQ=255)                                                                                  cgcCCGTTATGTGGTGCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTAtt                          >  1:503569/1‑133 (MQ=255)                                                                                                 gCTGGCAGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCAt           <  2:503569/133‑1 (MQ=255)                                                                                                    ggCAGAAGGTCCCAAGAGCGTCCTGGATTAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTg        <  2:245753/133‑1 (MQ=255)                                                                                                       aGAAGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGACCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGgag     <  1:7911/133‑1 (MQ=255)                                                                                                          aGGCGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAAt  <  1:189115/133‑1 (MQ=255)                                                                                                             |                                                                                                                                  CATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAAT  >  NC_000913/2893013‑2893250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑