Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,416	T→C	noncoding (109/369 nt)	csrB ←	small regulatory RNA CsrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,416	0	T	C	100.0%	201.7 / NA	57	noncoding (109/369 nt)	csrB	small regulatory RNA CsrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/20);  total (37/20)

TTGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTC  >  NC_000913/2924288‑2924539                                                                                                                                 |                                                                                                                            ttGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATc                                                                                                                         >  1:11343/1‑133 (MQ=255)  tGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATcc                                                                                                                        >  1:273567/1‑133 (MQ=255)      tCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCAATCCTGGATGTGCCCTTTAACGCGTACTGCGCCATCCTCtt                                                                                                                    >  2:152416/1‑133 (MQ=255)        cTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCg                                                                                                                  <  2:385887/133‑1 (MQ=255)         tGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGc                                                                                                                 >  1:33752/1‑133 (MQ=255)         tGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGc                                                                                                                 >  2:287314/1‑133 (MQ=255)                accaccCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATcc                                                                                                          >  1:505028/1‑133 (MQ=255)                     cccGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAg                                                                                                     >  1:82137/1‑133 (MQ=255)                     cccGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAg                                                                                                     >  2:16435/1‑133 (MQ=255)                          tgtgATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCttt                                                                                                >  1:51427/1‑133 (MQ=255)                          tgtgATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCttt                                                                                                <  2:53790/133‑1 (MQ=255)                               tACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGGTTCATCTAGAAGCCTTTCCCTg                                                                                           >  1:230710/1‑133 (MQ=255)                               tACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTg                                                                                           >  2:338992/1‑133 (MQ=255)                                aCTTCATCCTAAAGTCTCCCTGGCATTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGa                                                                                          <  2:160713/133‑1 (MQ=255)                                  ttCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGaaa                                                                                        <  1:16435/133‑1 (MQ=255)                                    cATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAaca                                                                                      <  2:448526/133‑1 (MQ=255)                                    cATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAaca                                                                                      <  2:475376/133‑1 (MQ=255)                                      tCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACAcc                                                                                    >  1:380038/1‑133 (MQ=255)                                         tGAAGTGTCCTTGGCTTTCCTGACCCACGGAATCATCCTGAACGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATc                                                                                 <  2:167402/133‑1 (MQ=255)                                              tgtCCCTGTCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGgt                                                                            <  1:152416/133‑1 (MQ=255)                                               gtCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCGGAAGCCTTTCCATGCAACACCATCCTGgtg                                                                           >  1:232259/1‑133 (MQ=255)                                                tCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGgtgt                                                                          >  1:509096/1‑133 (MQ=255)                                                 cccTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGgtgtg                                                                         <  1:77145/133‑1 (MQ=255)                                                     ggCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCt                                                                     >  2:330961/1‑133 (MQ=255)                                                     ggCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCt                                                                     >  2:93971/1‑133 (MQ=255)                                                      gCCTTCCTGACCCACCGAATCATCTTGAGCGGTTCTCATTCCCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTg                                                                    <  2:140304/133‑1 (MQ=255)                                                           ccTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCTTGAAACAGCATACTAGTGTGTACTGCAGaa                                                               >  1:140304/1‑133 (MQ=255)                                                           ccTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGgtgtg                                                                         >  1:116935/1‑123 (MQ=255)                                                           ccTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGgtgtg                                                                         <  2:116935/123‑1 (MQ=255)                                                           ccTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGaa                                                               <  1:287314/133‑1 (MQ=255)                                                            cTGACCCAGCGAATCGTCGTGATCGGTTCTAATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAg                                                              <  1:241352/133‑1 (MQ=255)                                                            cTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAg                                                              <  2:51427/133‑1 (MQ=255)                                                            cTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAg                                                              <  1:338992/133‑1 (MQ=255)                                                                    ccGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGtcatca                                                      >  1:439326/1‑133 (MQ=255)                                                                    ccGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGtcatca                                                      >  1:429935/1‑133 (MQ=255)                                                                     cGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCTTCCTGGTGTGTACTGCAGAAGGGTcatcat                                                     >  1:135026/1‑133 (MQ=255)                                                                           atcCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGAt                                               >  2:333008/1‑133 (MQ=255)                                                                                 aCCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCAc                                         >  1:405593/1‑133 (MQ=255)                                                                                  ccGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACt                                        >  2:479071/1‑133 (MQ=255)                                                                                      ttCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGt                                    >  2:93029/1‑133 (MQ=255)                                                                                      ttCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGt                                    >  1:40112/1‑133 (MQ=255)                                                                                                  aTCCTGGAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTccc                        <  1:270733/133‑1 (MQ=255)                                                                                                    tcctgAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTg                      >  1:73982/5‑133 (MQ=255)                                                                                                       ggAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCTTCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcg                   >  2:8524/1‑133 (MQ=255)                                                                                                       ggAGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcg                   >  2:371658/1‑133 (MQ=255)                                                                                                         aGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcgac                 <  2:273567/133‑1 (MQ=255)                                                                                                         aGGTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcgac                 <  2:509096/133‑1 (MQ=255)                                                                                                          ggTGTCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcgacg                <  2:505028/133‑1 (MQ=255)                                                                                                             gtCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCATTTCGTTGTCTGACTCCCTGTCGACGAAg             >  2:201151/1‑133 (MQ=255)                                                                                                             gtCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGCCTCCCTGTCGACGAAg             >  2:281931/1‑133 (MQ=255)                                                                                                             gtCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAg             >  2:507025/1‑133 (MQ=255)                                                                                                              tCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGa            >  1:320307/1‑133 (MQ=255)                                                                                                              tCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGa            <  1:330961/133‑1 (MQ=255)                                                                                                              tCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGa            >  2:102543/1‑133 (MQ=255)                                                                                                              tCCTTTAACGCGTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTACACATAGTTGTCTGACCCCCTGTCGACGAAGa            >  2:236809/1‑133 (MQ=255)                                                                                                                        cgTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTc  >  1:311738/1‑133 (MQ=255)                                                                                                                        cgTCCTGCGCCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTc  >  1:75162/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                            TTGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTC  >  NC_000913/2924288‑2924539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑