Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,585	G→A	A99T (GCC→ACC)	yaeQ →	uncharacterized protein YaeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,585	0	G	A	100.0%	46.6 / NA	15	A99T (GCC→ACC)	yaeQ	uncharacterized protein YaeQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

TACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGGCCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACT  >  NC_000913/214461‑214706                                                                                                                             |                                                                                                                          tACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGaa                                                                                                                   >  2:64259/1‑133 (MQ=255)   cccGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGt                                                                                                                 >  2:432985/1‑133 (MQ=255)     cGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTgg                                                                                                               <  1:291505/133‑1 (MQ=255)       tGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGgcg                                                                                                             <  1:64259/133‑1 (MQ=255)           ttGTGTGCCGATGATTAGTCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGt                                                                                                         <  1:206224/133‑1 (MQ=255)             gtgtgCCGATTATGAACCGGAAGCGTGGCTGCCTAACGATCCCCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGttt                                                                                                       <  2:133191/133‑1 (MQ=255)                  ccGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCta                                                                                                  >  2:83593/1‑133 (MQ=255)                             ccGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTACCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCggg                                                                                       >  1:114909/1‑133 (MQ=255)                                                      aCCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCaaaa                                                              >  2:22509/1‑133 (MQ=255)                                                                      gtgGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAAtgtgtg                                              <  2:287759/133‑1 (MQ=255)                                                                                cTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCa                                    >  1:124070/1‑133 (MQ=255)                                                                                   gggCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCAATc                                 >  1:299263/1‑133 (MQ=255)                                                                                         ccGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTAGGGCGGCGCAACTCTGGTGGCAGCAAAATCAAAGGAAAAGTGTGCAGATTGCGAATCTTTCCg                           >  2:157018/1‑133 (MQ=255)                                                                                                                 gCCTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCAATCTTTCCGTCTGGTACCTGGACGATGaacaac   >  1:382836/1‑133 (MQ=255)                                                                                                                  ccTGCACCCAGACCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCAATCTTTCCGTCTGGTACCTGGACGATGAACAACt  >  1:479635/1‑133 (MQ=255)                                                                                                                             |                                                                                                                          TACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTGTGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGGCCGCAGAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAGAGCAAATGTGTGCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACT  >  NC_000913/214461‑214706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑