| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,944,020 | T→C | G228G (GGT→GGC) | csdA → | cysteine sulfinate desulfinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,944,020 | 0 | T | C | 85.7% | 31.2 / ‑1.5 | 14 | G228G (GGT→GGC) | csdA | cysteine sulfinate desulfinase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (7/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTGGAAACTGGAA > NC_000913/2943897‑2944146 | ttCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTg < 2:266459/133‑1 (MQ=255) aGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTg < 1:261224/133‑1 (MQ=255) gatggttgatggtGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGaa < 1:368309/133‑1 (MQ=255) atggtGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGa > 1:338717/1‑133 (MQ=255) gggCAGTGCATTTCCCCGTGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGcc < 1:201430/133‑1 (MQ=255) cATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCctggctgg > 1:329279/1‑133 (MQ=255) tCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGggcgg < 2:338717/133‑1 (MQ=255) aCTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGTCCTAGATCGGCGGCGGAAAAAAGGTTCACGaa > 2:252580/1‑133 (MQ=255) gATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGa > 2:115255/1‑133 (MQ=255) tGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTc > 2:286864/1‑133 (MQ=255) tttCAGGTCACAAACTGTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGAc < 2:329279/133‑1 (MQ=255) gTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTgg > 1:386020/1‑133 (MQ=255) gTATGGCCCGACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTgg > 2:30708/1‑133 (MQ=255) gACAGGCATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTAACGACTCCATCCGCCCCGTGGAAACTGGaa > 1:237947/1‑133 (MQ=255) | TTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTGGAAACTGGAA > NC_000913/2943897‑2944146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |