Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,963,665	C→T	A18T (GCG→ACG)	ppdB ←	conserved protein PpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,963,665	0	C	T	100.0%	36.9 / NA	12	A18T (GCG→ACG)	ppdB	conserved protein PpdB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

TTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGCCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGTCTTCCCCACGGCGAAACCACCAGCCACCACTCGCCCGTTGAGTT  >  NC_000913/2963567‑2963793                                                                                                   |                                                                                                                                 ttCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCt                                                                                                <  2:410675/133‑1 (MQ=255)      cTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTTCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGctc                                                                                           <  1:464087/133‑1 (MQ=255)                cAGCTTACGGGTGCTAGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGc                                                                                 <  1:202663/133‑1 (MQ=255)                 aGCTTACGCGTGCTCATTGAACTTTCACGCGGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCa                                                                                <  1:186875/133‑1 (MQ=255)                     tACGGGTGCTCGTTAAACTTTCACGCTGGAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCACCCt                                                                            <  2:479853/133‑1 (MQ=255)                             cTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTtc                                                                    >  2:25302/1‑133 (MQ=255)                                  tAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCtt                                                               >  2:275669/1‑133 (MQ=255)                                                   aCGCAGGCCGAAAGCTTGCCGCCCCCAGCACCAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGt                                              <  1:234506/133‑1 (MQ=255)                                                           agAAAGCGTGCCGCCCCCGGCAAAAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCCTCCTTCTGTGTCTACATAGTGACAAAGCCGGATTCTTcccaa                                      >  2:250609/1‑131 (MQ=255)                                                           agAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTTTCCTTGCGGACAAAGCCGGAGTCTTcccaa                                      >  1:158552/1‑131 (MQ=255)                                                                   tGCCGCCCCCAGCAACAATACGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGTCTTCCCCACGGCGaaa                              >  1:91149/1‑133 (MQ=255)                                                                         caccAGCAACAATACACTCCTGATAGTCATAGAAATCAGAACTTCCAGAAGAGAAAAACCTTGCTCTTTTACAGGCATACTTCTGTTTCTCCTAGCTGACAAAGCCGGAGCCTTCCCCAAGGCGAAAccacca                        >  2:388592/3‑133 (MQ=255)                                                                                       cGCTACTGATCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGTCTTCCGCACGGCGGAACTACTAGCCACTACTCGccc          >  1:240499/1‑133 (MQ=255)                                                                                               aTCGTCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGTCTTCCCCACGGCGAAACCACCAGCCACCACTCGCCCGTTGAGtt  >  1:335470/1‑133 (MQ=255)                                                                                                   |                                                                                                                                 TTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCGTGCCGCCCCCAGCAACAATACGCTACTGATCGCCATAGCAATCAACACTTCCAGCAGAGAAAAACCTTGCTCTTTTACAGGCATCCTTCTGTTTCTCCTTGCTGACAAAGCCGGAGTCTTCCCCACGGCGAAACCACCAGCCACCACTCGCCCGTTGAGTT  >  NC_000913/2963567‑2963793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑