Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,033,402	T→C	V113A (GTG→GCG)	idi →	isopentenyl‑diphosphate Delta‑isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,033,402	0	T	C	100.0%	138.3 / NA	40	V113A (GTG→GCG)	idi	isopentenyl‑diphosphate Delta‑isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

GAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACG  >  NC_000913/3033282‑3033526                                                                                                                         |                                                                                                                             gagaAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGCGTGACACTGCGGAAAATGAAgt                                                                                                                  >  1:238132/1‑133 (MQ=255) gagaAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAgt                                                                                                                  <  1:90633/133‑1 (MQ=255) gagaAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAgt                                                                                                                  >  1:514751/1‑133 (MQ=255) gagaAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGAAAAATGAAgt                                                                                                                  >  2:112827/1‑133 (MQ=255)                   cAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCcg                                                                                                 <  2:515769/132‑1 (MQ=255)                   cAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCcg                                                                                                 >  1:515769/1‑132 (MQ=255)                    actGATCCGGGGTTGCCGTTATGAGCCTCGCGTGGAAATTATGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCcgca                                                                                               <  2:238132/131‑1 (MQ=255)                    aGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCcgca                                                                                               <  1:272928/133‑1 (MQ=255)                      tGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCcgcacg                                                                                             <  2:514751/133‑1 (MQ=255)                          ccgccgTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcacc                                                                                         >  2:332466/1‑133 (MQ=255)                            gccgttgccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac                                                                                       <  2:365624/133‑1 (MQ=255)                            gccgttgccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac                                                                                       <  1:306987/133‑1 (MQ=255)                            gccgttgccgctATGCGCTTGGCGTGGAAATTCCGCCTCCTCAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac                                                                                       <  2:140607/133‑1 (MQ=255)                            gccgttgccgctATGAGCTTGGCGGGGAAATTCCGCCTCCTCAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac                                                                                       <  2:176739/133‑1 (MQ=255)                              cgttgccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTa                                                                                     >  1:282441/1‑133 (MQ=255)                                 tgccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTGTCTATCCTGACTTTCGCTACCGCGCCGCCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCAACACTAGTg                                                                                  <  2:100281/133‑1 (MQ=255)                                 tgccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTg                                                                                  <  2:437446/133‑1 (MQ=255)                                  gccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGACAATGAAGTTTGTTCGGATTTTGCCGCACGCAGCACTAGTGc                                                                                 >  2:241413/1‑133 (MQ=255)                                  gccgttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGc                                                                                 >  1:416371/1‑133 (MQ=255)                                     gttATGAGCTTGGCGCGGAAATTAGGCCCCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGtt                                                                              <  2:201946/133‑1 (MQ=255)                                      ttATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTa                                                                             <  2:104876/133‑1 (MQ=255)                                          gAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGa                                                                         <  2:429940/133‑1 (MQ=255)                                             cTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCa                                                                      >  1:256003/1‑133 (MQ=255)                                              ttGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCaa                                                                     <  1:16181/133‑1 (MQ=255)                                                  cGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAatgat                                                                 <  1:57207/133‑1 (MQ=255)                                                      gAAATTACGCGTCCTTAAACTATCCATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTATCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAatgatgatg                                                             <  2:195040/133‑1 (MQ=255)                                                        aaTTACGCCTCCTGAATCTATCTAGCCTTACTATCGCTACCCCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGaa                                                           <  2:179980/133‑1 (MQ=255)                                                            aCGCCTCCCGAATCTCTCTATCTTGACTTTTGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGa                                                       <  1:151113/133‑1 (MQ=255)                                                                      aaTCTATCGATCCTGACTATCGCTACCGCGTCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCa                                             <  1:241413/133‑1 (MQ=255)                                                                       atctatctATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCaa                                            <  2:260103/133‑1 (MQ=255)                                                                            tctatcCTCACTTTCGCTGCCGGGCCACCGTTCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGgtg                                       <  1:243160/133‑1 (MQ=255)                                                                                tcCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGAt                                   >  1:509690/1‑133 (MQ=255)                                                                                     aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGc                              >  2:471857/1‑133 (MQ=255)                                                                                     aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGc                              >  2:462447/1‑133 (MQ=255)                                                                                     aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGc                              >  2:430871/1‑133 (MQ=255)                                                                                     aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGc                              <  1:332466/133‑1 (MQ=255)                                                                                       tttCGCTACCTCGCCACCGATCCAAGGGGCATTGCGGAAAATGAAGTGTGTCCGGGATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTATTGGATTATCAATGGTGTGATTTAGCAg                            <  2:248196/133‑1 (MQ=255)                                                                                       tttCGCTACCGCGCCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAg                            <  1:110962/133‑1 (MQ=255)                                                                                                 gcgcCACCGATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGAGTGGTTAAGCAGAAGTATTaca                  >  1:141103/1‑133 (MQ=255)                                                                                                  cgcCACCGATTCGAGCGGCATTGCTGAATATGAAGTGTGTCAGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTacac                 <  2:171767/133‑1 (MQ=255)                                                                                                         gATCCGAGTGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATAGTGTGATTTAGCAGATTTATTACACGGTATTg          >  2:185069/1‑133 (MQ=255)                                                                                                                 tGGCATTGCGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCAca  >  2:108453/1‑132 (MQ=255)                                                                                                                         |                                                                                                                             GAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACG  >  NC_000913/3033282‑3033526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑